PMID- 19439121
OWN - NLM
STAT- MEDLINE
DCOM- 20100511
LR  - 20090514
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 21
IP  - 5
DP  - 2009 May
TI  - [Preliminary study of skeletal muscle apoptosis after severe thermal injury in 
      rats].
PG  - 304-6
AB  - OBJECTIVE: To investigate changes in skeletal muscle apoptosis after a severe 
      thermal injury in rats. METHODS: One hundred Wistar rats were randomly divided 
      into two groups: sham thermal injury group and severe thermal injury group. They 
      were subdivided into 1, 4, 7, 10, 14 days post-injury with 10 rats in each 
      subgroup. Rats in severe thermal injury group were subjected to a 40% total body 
      surface area full-thickness scald injury. Both weight and tibialis anterior (TA) 
      mass of rats were weighed on 1, 4, 7, 10, 14 days post-injury. Electron 
      microscope was used for observing ultrastructural changes in skeletal muscle, 
      including apoptosis. Tissues of tibialis anterior from burn and sham burn animals 
      were then examined by the terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) assay and immunohistochemistry. RESULTS: Compared with 
      sham thermal injury group, body weight and TA mass of rats were decreased from 
      first day on, and it dropped to the lowest level at 4 days (P<0.05 and P<0.01), 
      and started to regain from 7 days on (all P<0.01). Electron micrographs showed 
      condensation of chromatin around the periphery of the nucleus, blebbing of the 
      sarcolemma, and free of myofibrils near myonuclei in a large area in skeletal 
      muscle of thermally injured rats. Sporadic TUNEL positive myonuclei were also 
      seen under light microscope in skeletal muscle in thermal injury group. There 
      were no characteristic signs of apoptosis in skeletal muscle in rats of sham 
      group. CONCLUSION: There are skeletal muscle apoptosis after severe thermal 
      injury. It may contribute to atrophy of skeletal muscle after burn injury in 
      rats.
FAU - Duan, Hong-jie
AU  - Duan HJ
AD  - Department of Burns and Plastic Surgery, Burns Institute, First Affiliated 
      Hospital of PLA, Beijing 100048, China.
FAU - Chai, Jia-ke
AU  - Chai JK
FAU - Yao, Yong-ming
AU  - Yao YM
FAU - Yin, Hui-nan
AU  - Yin HN
FAU - Shen, Chuan-an
AU  - Shen CA
FAU - Wu, Yan-qiu
AU  - Wu YQ
FAU - Lin, Jing
AU  - Lin J
FAU - Liang, Li-ming
AU  - Liang LM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Burns/*pathology
MH  - Cell Nucleus/ultrastructure
MH  - Disease Models, Animal
MH  - Muscle, Skeletal/*pathology/ultrastructure
MH  - Rats
MH  - Rats, Wistar
EDAT- 2009/05/15 09:00
MHDA- 2010/05/12 06:00
CRDT- 2009/05/15 09:00
PHST- 2009/05/15 09:00 [entrez]
PHST- 2009/05/15 09:00 [pubmed]
PHST- 2010/05/12 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 May;21(5):304-6.