PMID- 19440251
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20211020
IS  - 1874-2858 (Electronic)
IS  - 1874-2858 (Linking)
VI  - 3
DP  - 2009
TI  - Regulation of clpQ(+)Y(+) (hslV(+)U(+)) gene expression in Escherichia coli.
PG  - 29-39
LID - 10.2174/1874285800903010029 [doi]
AB  - The Escherichia coli ClpYQ (HslUV) complex is an ATP-dependent protease, and the 
      clpQ(+)Y(+) (hslV(+)U(+)) operon encodes two heat shock proteins, ClpQ and ClpY, 
      respectively. The transcriptional (op) or translational (pr) clpQ(+)::lacZ fusion 
      gene was constructed, with the clpQ(+)Y(+) promoter fused to a lacZ reporter gene. 
      The clpQ(+)::lacZ (op or pr) fusion gene was each crossed into lambda phage. The 
      lambdalpQ(+)::lacZ(+) (op), a transcriptional fusion gene, was used to form lysogens in 
      the wild-type, rpoH or/and rpoS mutants. Upon shifting the temperature up from 30 
       degrees  C to 42  degrees  C, the wild-type lambdaclpQ(+)::lacZ(+) (op) demonstrates an increased 
      beta-galactosidase (betaGal) activity. However, the betaGal activity of clpQ(+)::lacZ(+) (op) 
      was decreased in the rpoH and rpoH rpoS mutants but not in the rpoS mutant. The 
      levels of clpQ(+)::lacZ(+) mRNA transcripts correlated well to their betaGal activity. 
      Similarly, the expression of the clpQ(+)::lacZ(+) gene fusion was nearly identical to 
      the clpQ(+)Y(+) transcript under the in vivo condition. The clpQ(m1)::lacZ(+), 
      containing a point mutation in the -10 promoter region for RpoH binding, showed 
      decreased betaGal activity, independent of activation by RpoH. We conclude that RpoH 
      itself regulates clpQ(+)Y(+) gene expression. In addition, the clpQ(+)Y(+) message 
      carries a conserved 71 bp at the 5' untranslated region (5'UTR) that is predicted 
      to form the stem-loop structure by analysis of its RNA secondary structure. The 
      clpQ(m2)Delta40::lacZ(+), with a 40 bp deletion in the 5'UTR, showed a decreased betaGal 
      activity. In addition, from our results, it is suggested that this stem-loop 
      structure is necessary for the stability of the clpQ(+)Y(+) message.
FAU - Lien, Hsiang-Yun
AU  - Lien HY
AD  - Department of Agricultural Chemistry, National Taiwan University, Taipei (106), 
      Taiwan, R.O.C.
FAU - Yu, Chien-Hung
AU  - Yu CH
FAU - Liou, Chung-Ming
AU  - Liou CM
FAU - Wu, Whi Fin
AU  - Wu WF
LA  - eng
PT  - Journal Article
DEP - 20090317
PL  - United Arab Emirates
TA  - Open Microbiol J
JT  - The open microbiology journal
JID - 101480482
PMC - PMC2681174
OTO - NOTNLM
OT  - 5' stem-loop structure
OT  - ATP-dependent protease
OT  - ClpYQ (HslUV)
OT  - E. coli
OT  - Gene regulation
OT  - Promoter activity
OT  - mRNA stability.
EDAT- 2009/05/15 09:00
MHDA- 2009/05/15 09:01
PMCR- 2009/01/01
CRDT- 2009/05/15 09:00
PHST- 2009/02/11 00:00 [received]
PHST- 2009/02/17 00:00 [revised]
PHST- 2009/02/24 00:00 [accepted]
PHST- 2009/05/15 09:00 [entrez]
PHST- 2009/05/15 09:00 [pubmed]
PHST- 2009/05/15 09:01 [medline]
PHST- 2009/01/01 00:00 [pmc-release]
AID - TOMICROJ-3-29 [pii]
AID - 10.2174/1874285800903010029 [doi]
PST - ppublish
SO  - Open Microbiol J. 2009;3:29-39. doi: 10.2174/1874285800903010029. Epub 2009 Mar 
      17.