PMID- 19440697
OWN - NLM
STAT- MEDLINE
DCOM- 20090819
LR  - 20211020
IS  - 1432-1912 (Electronic)
IS  - 0028-1298 (Linking)
VI  - 380
IP  - 2
DP  - 2009 Aug
TI  - Suppressive effect of CTB glycoprotein (75 kDa) on IL-4 expression in 
      primary-cultured lymphocytes treated with di(2-ethylhexyl) phthalate.
PG  - 115-24
LID - 10.1007/s00210-009-0423-y [doi]
AB  - The purpose of this study is to determine the inhibitory effect of a glycoprotein 
      isolated from Cudrania tricuspidata Bureau (CTB glycoprotein, 75 kDa) on 
      di(2-ethylhexyl) phthalate (DEHP)-induced differentiation of T helper (Th) type 2 
      cells in T lymphocytes separated from mice. This experiment evaluated the 
      activities of protein kinase C (PKC), mitogen-activated protein kinase (MAPK), 
      transcription factors [signal transducer and activator of transcription (STAT)-6 
      and GATA-binding protein 3 (GATA3)], and Th2 cell-related cytokine [interleukin-4 
      (IL-4)] using immunoblotting and reverse transcription-polymerase chain reaction. 
      Our results revealed that the CTB glycoprotein in the presence of DEHP inhibits 
      the translocation of PKC from cytosol to membrane and the phosphorylation of 
      p44/42 MAPK in primary cultured T lymphocytes. We also found that the CTB 
      glycoprotein (100 microg/ml) has suppressive effects on transcriptional 
      activation of STAT6, GATA3, and on the expression level of IL-4 in DEHP-treated T 
      lymphocytes. The phosphorylation of STAT6 and GATA3 were collectively blocked by 
      treatment with PKC inhibitor (staurosporine) as well as p44/42 MAPK inhibitor (PD 
      98059), respectively. The results from these experiments indicated that the CTB 
      glycoprotein inhibits IL-4 expression, not IL-10 expression, on the stage of Th2 
      cell differentiation induced by DEHP in T lymphocytes. Hence, we speculate that 
      the CTB glycoprotein has a strong inhibitory ability for expressions of 
      allergy-related cytokines indirectly caused by DEHP in Th2 cell differentiation 
      of the primary cultured mouse T lymphocytes.
FAU - Oh, Phil-Sun
AU  - Oh PS
AD  - Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for 
      the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National 
      University, Gwang-ju, South Korea.
FAU - Lim, Kye-Taek
AU  - Lim KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090514
PL  - Germany
TA  - Naunyn Schmiedebergs Arch Pharmacol
JT  - Naunyn-Schmiedeberg's archives of pharmacology
JID - 0326264
RN  - 0 (Glycoproteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Diethylhexyl Phthalate/pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Glycoproteins/isolation & purification/*pharmacology
MH  - Interleukin-10/genetics
MH  - Interleukin-4/*genetics
MH  - Medicine, Korean Traditional
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Moraceae/*chemistry
MH  - Phosphorylation/drug effects
MH  - Protein Kinase C/drug effects/metabolism
MH  - Protein Transport/drug effects
MH  - T-Lymphocytes/drug effects/metabolism
MH  - Th2 Cells/drug effects/metabolism
EDAT- 2009/05/15 09:00
MHDA- 2009/08/20 09:00
CRDT- 2009/05/15 09:00
PHST- 2009/02/23 00:00 [received]
PHST- 2009/04/20 00:00 [accepted]
PHST- 2009/05/15 09:00 [entrez]
PHST- 2009/05/15 09:00 [pubmed]
PHST- 2009/08/20 09:00 [medline]
AID - 10.1007/s00210-009-0423-y [doi]
PST - ppublish
SO  - Naunyn Schmiedebergs Arch Pharmacol. 2009 Aug;380(2):115-24. doi: 
      10.1007/s00210-009-0423-y. Epub 2009 May 14.