PMID- 194417
OWN - NLM
STAT- MEDLINE
DCOM- 19770729
LR  - 20061115
IS  - 0042-8809 (Print)
IS  - 0042-8809 (Linking)
VI  - 22
IP  - 6
DP  - 1976 Nov-Dec
TI  - [Subcellular distribution of hexokinase in hepatoma and its electrophoretic 
      properties].
PG  - 798-804
AB  - In hepatomas of various malignancy contrary to normal and regenerating liver 
      tissue a considerable part of hexokinase (HK) activity was bound with 
      mitochondria. The HK activity, estimated in nuclei, microsomes and apparently in 
      cytoplasma membranes, was due to contamination with mitochondrial fraction. High 
      specific activity of HK in mitochondria of tumors might be responsible for the 
      increased glycolysis, when these organelle preparations were added to the soluble 
      fraction of cells. The HK of tumors was extracted from the preparations by 
      alternative pathways. Reversible binding of some part of the enzyme with 
      mitochondria was regulated by concentration of metabolites (ATP and G6P) 
      similarly to the bound HK from normal tissues (brain, retina). At the same time 
      the other part of unregulated HK activity (isozyme I only) was found in hepatomas 
      to be intercalated into lipoprotein membranes; it was not controlled by 
      metabolites.
FAU - Gobeev, V N
AU  - Gobeev VN
FAU - Khripach, L V
AU  - Khripach LV
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Subkletochnoe raspredelenie geksokinazy v perevivaemykh gepatomakh i ee 
      elektroforeticheskie svoistva.
PL  - Russia (Federation)
TA  - Vopr Med Khim
JT  - Voprosy meditsinskoi khimii
JID - 0416601
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular/*pathology
MH  - Cell Membrane/analysis/enzymology
MH  - Cell Nucleus/*enzymology
MH  - Electron Transport Complex IV/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glycolysis
MH  - Hexokinase/analysis/*metabolism
MH  - Liver Neoplasms/*pathology
MH  - Mice
MH  - Microsomes/*enzymology
MH  - Mitochondria, Liver/analysis/*enzymology
MH  - Neoplasms, Experimental/pathology
EDAT- 1976/11/01 00:00
MHDA- 1976/11/01 00:01
CRDT- 1976/11/01 00:00
PHST- 1976/11/01 00:00 [pubmed]
PHST- 1976/11/01 00:01 [medline]
PHST- 1976/11/01 00:00 [entrez]
PST - ppublish
SO  - Vopr Med Khim. 1976 Nov-Dec;22(6):798-804.