PMID- 19442647
OWN - NLM
STAT- MEDLINE
DCOM- 20090910
LR  - 20111117
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 391
IP  - 2
DP  - 2009 Aug 15
TI  - An automated microfluidic-based immunoassay cartridge for allergen screening and 
      other multiplexed assays.
PG  - 98-105
LID - 10.1016/j.ab.2009.05.009 [doi]
AB  - A microfluidic cartridge and system for multiplexed immunoassays is described. 
      The passive microfluidic cartridge was composed of three layers of injection 
      molded plastic sealed together using a thermal staking technique. Using this 
      platform technology, a specific immunoglobulin E (IgE) panel assay was 
      constructed. Allergen extract targets, positive and negative controls, and IgE 
      calibration standards were immobilized within the cartridge as a microarray. A 
      computer-controlled solenoid array provided the necessary actuation force for 
      pumping reagents within the cartridge to perform an automated, chemiluminescent 
      indirect immunoassay. A 20-target allergen extract panel was demonstrated on the 
      device with a total analysis time of 27 min. Allergen screening results showed 
      84% agreement for 3 house dust mites (N=300) compared with a commercial test and 
      80% agreement overall (N=978). Average coefficients of variation (N=80) were 
      measured as 20.5% for low/medium levels and 20.4% for medium/high levels. The 
      average limit of detection (N=160) was measured at 0.535 AU, and cutoff levels of 
      1.0 AU were estimated at less than 1 IU/ml (2.4 ng/ml). Such a system has 
      potential applications in decentralized allergen screening as well as in other 
      near-patient diagnostic immunoassays where multiplexed analysis, ease of use, and 
      short analysis time are critical.
FAU - Tai, Lung-Wen
AU  - Tai LW
AD  - Agnitio Science and Technology, Hsinchu 30075, Taiwan.
FAU - Tseng, Ko-Yuan
AU  - Tseng KY
FAU - Wang, Shaw-Tsu
AU  - Wang ST
FAU - Chiu, Chao-Cheng
AU  - Chiu CC
FAU - Kow, Chak Hone
AU  - Kow CH
FAU - Chang, Ping
AU  - Chang P
FAU - Chen, Chichen
AU  - Chen C
FAU - Wang, Jiu-Yao
AU  - Wang JY
FAU - Webster, James R
AU  - Webster JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090512
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*analysis/immunology
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/analysis/immunology
MH  - Luminescent Measurements
MH  - Microfluidics/*instrumentation/methods
MH  - Reproducibility of Results
EDAT- 2009/05/16 09:00
MHDA- 2009/09/11 06:00
CRDT- 2009/05/16 09:00
PHST- 2009/03/13 00:00 [received]
PHST- 2009/04/23 00:00 [revised]
PHST- 2009/05/06 00:00 [accepted]
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/09/11 06:00 [medline]
AID - S0003-2697(09)00325-X [pii]
AID - 10.1016/j.ab.2009.05.009 [doi]
PST - ppublish
SO  - Anal Biochem. 2009 Aug 15;391(2):98-105. doi: 10.1016/j.ab.2009.05.009. Epub 2009 
      May 12.