PMID- 19442818
OWN - NLM
STAT- MEDLINE
DCOM- 20090804
LR  - 20220408
IS  - 1872-9711 (Electronic)
IS  - 0161-813X (Print)
IS  - 0161-813X (Linking)
VI  - 30
IP  - 3
DP  - 2009 May
TI  - Impact of neonatal exposure to the ERalpha agonist PPT, bisphenol-A or 
      phytoestrogens on hypothalamic kisspeptin fiber density in male and female rats.
PG  - 350-7
LID - 10.1016/j.neuro.2009.02.010 [doi]
AB  - Neonatal exposure to endocrine disrupting compounds (EDCs) can impair 
      reproductive physiology, but the specific mechanisms by which this occurs remain 
      largely unknown. Growing evidence suggests that kisspeptin (KISS) neurons play a 
      significant role in the regulation of pubertal onset and ovulation, therefore 
      disruption of KISS signaling could be a mechanism by which EDCs impair 
      reproductive maturation and function. We have previously demonstrated that 
      neonatal exposure to phytoestrogens decreases KISS fiber density in the anterior 
      hypothalamus of female rats, an effect which was associated with early persistent 
      estrus and the impaired activation gonadotropin releasing hormone (GnRH) neurons. 
      The goals of the present study were to (1) determine if an ERalpha selective 
      agonist (PPT) or bisphenol-A (BPA) could produce similar effects on hypothalamic 
      KISS content in female rats and (2) to determine if male KISS fiber density was 
      also vulnerable to disruption by EDCs. We first examined the effects of neonatal 
      exposure to PPT, a low (50 microg/kg bw) BPA dose, and a high (50 mg/kg bw) BPA 
      dose on KISS immunoreactivity (-ir) in the anterior ventral periventricular 
      (AVPV) and arcuate (ARC) nuclei of adult female rats, using estradiol benzoate 
      (EB) and a sesame oil vehicle as controls. AVPV KISS-ir, following ovariectomy 
      (OVX) and hormone priming, was significantly lower in the EB and PPT groups but 
      not the BPA groups. ARC KISS-ir levels were significantly diminished in the EB 
      and high dose BPA groups, and there was a nonsignificant trend for lower KISS-ir 
      in the PPT group. We next examined effects of neonatal exposure to a low (50 
      microg/kg bw) dose of BPA and the phytoestrogens genistein (GEN) and equol (EQ) 
      on KISS-ir in the AVPV and ARC of adult male rats, using OVX females as an 
      additional control group. None of the compounds affected KISS-ir in the male 
      hypothalamus. Our results suggest that the organization of hypothalamic KISS 
      fibers may be vulnerable to disruption by EDC exposure and that females might be 
      more sensitive than males.
FAU - Patisaul, Heather B
AU  - Patisaul HB
AD  - Department of Biology, North Carolina State University, 127 David Clark Labs, 
      Raleigh, NC 27695, USA. Heather_Patisaul@ncsu.edu
FAU - Todd, Karina L
AU  - Todd KL
FAU - Mickens, Jillian A
AU  - Mickens JA
FAU - Adewale, Heather B
AU  - Adewale HB
LA  - eng
GR  - R01 ES016001/ES/NIEHS NIH HHS/United States
GR  - R01ES016001/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090225
PL  - Netherlands
TA  - Neurotoxicology
JT  - Neurotoxicology
JID - 7905589
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Kiss1 protein, rat)
RN  - 0 (Kisspeptins)
RN  - 0 (Phenols)
RN  - 0 (Phytoestrogens)
RN  - 0 (Proteins)
RN  - 0 (Pyrazoles)
RN  - 0T83Y6JZPF (4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol)
RN  - 1S4CJB5ZGN (estradiol 3-benzoate)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Animals
MH  - *Animals, Newborn
MH  - Benzhydryl Compounds
MH  - Estradiol/analogs & derivatives/pharmacology
MH  - Estrogen Receptor alpha/*agonists
MH  - Female
MH  - Hypothalamus/*anatomy & histology
MH  - Kisspeptins
MH  - Male
MH  - Nerve Fibers/*drug effects/metabolism
MH  - Ovariectomy
MH  - Phenols/*pharmacology
MH  - Phytoestrogens/*pharmacology
MH  - Progesterone/pharmacology
MH  - Proteins/metabolism
MH  - Pyrazoles/*pharmacology
MH  - Rats
MH  - Rats, Long-Evans
MH  - *Sex Characteristics
PMC - PMC3691558
MID - NIHMS479278
EDAT- 2009/05/16 09:00
MHDA- 2009/08/06 09:00
PMCR- 2013/06/25
CRDT- 2009/05/16 09:00
PHST- 2008/10/31 00:00 [received]
PHST- 2009/02/11 00:00 [revised]
PHST- 2009/02/13 00:00 [accepted]
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/08/06 09:00 [medline]
PHST- 2013/06/25 00:00 [pmc-release]
AID - S0161-813X(09)00050-3 [pii]
AID - 10.1016/j.neuro.2009.02.010 [doi]
PST - ppublish
SO  - Neurotoxicology. 2009 May;30(3):350-7. doi: 10.1016/j.neuro.2009.02.010. Epub 
      2009 Feb 25.