PMID- 19442857
OWN - NLM
STAT- MEDLINE
DCOM- 20090624
LR  - 20090515
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 159
IP  - 1
DP  - 2009 Jul
TI  - Length variability of telomeric repeat sequences of human herpesvirus 6 DNA.
PG  - 127-30
LID - 10.1016/j.jviromet.2009.03.002 [doi]
AB  - The telomeric repeat sequences (TRS) located near both ends of human herpesvirus 
      6 (HHV-6) genome are unique structures of unknown function among human 
      herpesviruses. The goal of the present study was to investigate the variability 
      of TRS copy number among different laboratory strains and HHV-6-infected clinical 
      specimens regarding the two variants A and B of HHV-6. DNA obtained from infected 
      cells was submitted to a PCR assay designed to amplify the part of genome 
      containing TRS specifically either for HHV-6A or HHV-6B. Amplicons were analyzed 
      by electrophoresis on agarose gel with ethidium bromide staining and nucleotide 
      sequencing. The number of TRS copies was highly variable among the distinct 
      laboratory strains and clinical specimens studied, ranging from 15 up to more 
      than 180. However, this number was constant for a given strain after serial 
      propagation in cell cultures as well as in different samples from the same 
      subject. This permitted to detect a mixed infection with two distinct strains of 
      HHV-6A within the same patient. The PCR-based analysis of HHV-6 TRS has a limited 
      sensitivity but is highly specific, which provides the opportunity to include it 
      in the set of molecular tools dedicated to the study of HHV-6 epidemiology.
FAU - Achour, Abla
AU  - Achour A
AD  - Laboratoire de Virologie, Universite Pierre et Marie Curie-Paris 6 EA2387, AP-HP, 
      Groupe Hospitalier Pitie-Salpetriere, Paris, France.
FAU - Malet, Isabelle
AU  - Malet I
FAU - Deback, Claire
AU  - Deback C
FAU - Bonnafous, Pascale
AU  - Bonnafous P
FAU - Boutolleau, David
AU  - Boutolleau D
FAU - Gautheret-Dejean, Agnes
AU  - Gautheret-Dejean A
FAU - Agut, Henri
AU  - Agut H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090314
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Cell Culture Techniques
MH  - DNA, Viral/*analysis
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Roseolovirus Infections/epidemiology/*virology
MH  - Sensitivity and Specificity
MH  - *Tandem Repeat Sequences
MH  - Telomere/*genetics
EDAT- 2009/05/16 09:00
MHDA- 2009/06/25 09:00
CRDT- 2009/05/16 09:00
PHST- 2008/12/19 00:00 [received]
PHST- 2009/02/25 00:00 [revised]
PHST- 2009/03/02 00:00 [accepted]
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/06/25 09:00 [medline]
AID - S0166-0934(09)00104-9 [pii]
AID - 10.1016/j.jviromet.2009.03.002 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Jul;159(1):127-30. doi: 10.1016/j.jviromet.2009.03.002. 
      Epub 2009 Mar 14.