PMID- 19443236
OWN - NLM
STAT- MEDLINE
DCOM- 20091006
LR  - 20211020
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Linking)
VI  - 20
IP  - 8
DP  - 2009 Aug
TI  - Screening and structural characterization of alpha-glucosidase inhibitors from 
      hawthorn leaf flavonoids extract by ultrafiltration LC-DAD-MS(n) and SORI-CID 
      FTICR MS.
PG  - 1496-503
LID - 10.1016/j.jasms.2009.04.003 [doi]
AB  - In vitro alpha-glucosidase inhibition assays and ultrafiltration liquid 
      chromatography with photodiode array detection coupled to electrospray ionization 
      tandem mass spectrometry (ultrafiltration LC-DAD-ESI-MS(n)) were combined to 
      screen alpha-glucosidase inhibitors from hawthorn leaf flavonoids extract (HLFE). 
      As a result, four compounds were identified as alpha-glucosidase inhibitors in 
      the HLFE, and their structures were confirmed to be quercetin-3-O-rha- 
      (1-4)-glc-rha and C-glycosylflavones (vitexin-2"-O-glucoside, 
      vitexin-2"-O-rhamnoside and vitexin) by high-resolution sustained off resonance 
      irradiation collision-induced dissociation (SORI-CID) data obtained by Fourier 
      transform ion cyclotron resonance mass spectrometry (FTICR MS). Several other 
      C-glycosylflavones (vitexin, isovitexin, orientin, isooriention) and their 
      aglycones apigenin and luteolin were evaluated by in vitro assays, and were found 
      to possess strong alpha-glucosidase inhibitory activities as well. Moreover, the 
      substituent groups on the flavones had a great impact on the enzyme inhibition 
      activity. C-3'-OH of the B-ring of flavones in particular increased the 
      alpha-glucosidase inhibition activity, whereas C-glycosylations at C-6 or C-8 of 
      the A ring weakened the inhibition activity.
FAU - Li, Huilin
AU  - Li H
AD  - Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 
      Center of Mass Spectrometry, Changchun, PR China.
FAU - Song, Fengrui
AU  - Song F
FAU - Xing, Junpeng
AU  - Xing J
FAU - Tsao, Rong
AU  - Tsao R
FAU - Liu, Zhiqiang
AU  - Liu Z
FAU - Liu, Shuying
AU  - Liu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090414
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Crataegus/*chemistry
MH  - *Glycoside Hydrolase Inhibitors
MH  - Mass Spectrometry/*methods
MH  - Plant Extracts/*chemistry
MH  - Plant Leaves/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared/*methods
MH  - Ultrafiltration/*methods
EDAT- 2009/05/16 09:00
MHDA- 2009/10/07 06:00
CRDT- 2009/05/16 09:00
PHST- 2008/11/25 00:00 [received]
PHST- 2009/04/03 00:00 [revised]
PHST- 2009/04/04 00:00 [accepted]
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/10/07 06:00 [medline]
AID - S1044-0305(09)00286-4 [pii]
AID - 10.1016/j.jasms.2009.04.003 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2009 Aug;20(8):1496-503. doi: 
      10.1016/j.jasms.2009.04.003. Epub 2009 Apr 14.