PMID- 19443644
OWN - NLM
STAT- MEDLINE
DCOM- 20090901
LR  - 20211020
IS  - 1533-3450 (Electronic)
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 20
IP  - 8
DP  - 2009 Aug
TI  - The CXCL12 (SDF-1)/CXCR4 axis is essential for the development of renal 
      vasculature.
PG  - 1714-23
LID - 10.1681/ASN.2008060640 [doi]
AB  - CXC chemokine ligand 12 (CXCL12; stromal cell-derived factor 1) is a unique 
      homeostatic chemokine that signals through its cognate receptor, CXCR4. 
      CXCL12/CXCR4 signaling is essential for the formation of blood vessels in the 
      gastrointestinal tract during development, but its contribution to renal 
      development remains unclear. Here, we found that CXCL12-secreting stromal cells 
      surround CXCR4-positive epithelial components of early nephrons and blood vessels 
      in the embryonic kidney. In glomeruli, we observed CXCL12-secreting podocytes in 
      close proximity to CXCR4-positive endothelial cells. Both CXCL12- and 
      CXCR4-deficient kidneys exhibited identical phenotypes; there were no apparent 
      abnormalities in early nephrogenesis or in differentiation of podocytes and 
      tubules, but there was defective formation of blood vessels, including ballooning 
      of the developing glomerular tuft and disorganized patterning of the renal 
      vasculature. To clarify the relative importance of different cellular defects 
      resulting from ablation of CXCL12 and CXCR4, we established endothelial 
      cell-specific CXCR4-deficient mice, which recapitulated the renal phenotypes of 
      conventional CXCR4-deficient mice. We conclude that CXCL12 secreted from stromal 
      cells or podocytes acts on endothelial cells to regulate vascular development in 
      the kidney. These findings suggest new potential therapeutic targets for 
      remodeling the injured kidney.
FAU - Takabatake, Yoshitsugu
AU  - Takabatake Y
AD  - Department of Geriatric Medicine and Nephrology, Osaka University Graduate School 
      of Medicine (B6), Suita, Japan. takaba@kid.med.osaka-u.ac.jp
FAU - Sugiyama, Tatsuki
AU  - Sugiyama T
FAU - Kohara, Hiroshi
AU  - Kohara H
FAU - Matsusaka, Taiji
AU  - Matsusaka T
FAU - Kurihara, Hidetake
AU  - Kurihara H
FAU - Koni, Pandelakis A
AU  - Koni PA
FAU - Nagasawa, Yasuyuki
AU  - Nagasawa Y
FAU - Hamano, Takayuki
AU  - Hamano T
FAU - Matsui, Isao
AU  - Matsui I
FAU - Kawada, Noritaka
AU  - Kawada N
FAU - Imai, Enyu
AU  - Imai E
FAU - Nagasawa, Takashi
AU  - Nagasawa T
FAU - Rakugi, Hiromi
AU  - Rakugi H
FAU - Isaka, Yoshitaka
AU  - Isaka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090514
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (CXCR4 protein, mouse)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Receptors, CXCR4)
SB  - IM
CIN - J Am Soc Nephrol. 2009 Aug;20(8):1659-61. PMID: 19608697
MH  - Animals
MH  - Chemokine CXCL12/*metabolism
MH  - Endothelial Cells/metabolism
MH  - Kidney/blood supply/*embryology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Podocytes/metabolism
MH  - Receptors, CXCR4/*metabolism
MH  - Stromal Cells/metabolism
PMC - PMC2723985
EDAT- 2009/05/16 09:00
MHDA- 2009/09/02 06:00
PMCR- 2010/08/01
CRDT- 2009/05/16 09:00
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/09/02 06:00 [medline]
PHST- 2010/08/01 00:00 [pmc-release]
AID - ASN.2008060640 [pii]
AID - 2008060640 [pii]
AID - 10.1681/ASN.2008060640 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2009 Aug;20(8):1714-23. doi: 10.1681/ASN.2008060640. Epub 2009 
      May 14.