PMID- 19443884
OWN - NLM
STAT- MEDLINE
DCOM- 20090715
LR  - 20141120
IS  - 1066-8969 (Print)
IS  - 1066-8969 (Linking)
VI  - 17
IP  - 3
DP  - 2009 Jun
TI  - Role of polysomy 17 in transitional cell carcinoma of the bladder: 
      immunohistochemical study of HER2/neu expression and fish analysis of c-erbB-2 
      gene and chromosome 17.
PG  - 198-205
LID - 10.1177/1066896909333415 [doi]
AB  - This study investigates the potential clinical significance of c-erbB-2 gene and 
      chromosome 17 alterations by fluorescence in situ hybridization (FISH) analysis 
      and HER2/neu overexpression by immunohistochemical staining in transitional cell 
      carcinoma (TCC) of urinary bladder correlating the results with tumor stage and 
      grade categories and with clinical behavior. Sixty-three cases of TCC retrieved 
      from the files of 2 institutions were analyzed for chromosome 17 aberrations and 
      c-erbB-2 amplification by FISH analysis and evaluated immunohistochemically for 
      HER2/neu overexpression. Five tumors were G1, 29 intermediate grade (G2), and 29 
      tumors high grade (G3); 32 tumors had stage Ta, 18 tumors T1, and 13 tumors T2. 
      We found polysomy of chromosome 17 in 58.7% of TCC with average chromosome copy 
      number >2.26; increased number of HER2/neu gene copy was observed in 66.7% of 
      tumors. C-erbB-2 amplification occurred in 6.3% of tumors. Immunohistochemically, 
      60.3% of TCC overexpressed HER2/neu and 39.7% of tumors were negative. All tumors 
      with polysomy showed simultaneously increase of HER2/neu gene copy number of 
      which 34/37 with protein overexpression. A statistically significant correlation 
      between polysomy of chromosome 17 and tumor stage (P = .0003) and tumor grade (P 
      < .0001) was found; polysomy was not seen in G1 tumors; however, 8/29 G2 tumors 
      and 29/29 G3 tumors revealed polysomy of chromosome 17; in 8/32 Ta tumors, 14/18 
      T1 and 13/13 of deeply invasive tumors (T2) polysomy 17 was observed. Moreover, 
      it was found that 7 superficial tumors (1 Ta and 6 T1) showed high polysomy with 
      average of chromosome 17 copy number > or =3.76 as observed in all invasive 
      tumors. The data suggest that although HER2/neu amplification, found in high 
      grade and invasive tumors, is a rare event in TCC, polysomy of chromosome 17 is 
      an important factor correlated with tumor stage and grade categories and could be 
      considered a molecular marker of tumor progression with interesting diagnostic 
      implications.
FAU - Simonetti, Sara
AU  - Simonetti S
AD  - Department of Biomorphological and Functional Sciences, University Federico II of 
      Naples, Naples, Italy.
FAU - Russo, Rosa
AU  - Russo R
FAU - Ciancia, Giuseppe
AU  - Ciancia G
FAU - Altieri, Vincenzo
AU  - Altieri V
FAU - De Rosa, Gaetano
AU  - De Rosa G
FAU - Insabato, Luigi
AU  - Insabato L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Surg Pathol
JT  - International journal of surgical pathology
JID - 9314927
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*genetics/pathology
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Female
MH  - Gene Amplification
MH  - Gene Dosage
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Receptor, ErbB-2/*biosynthesis/genetics
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2009/05/16 09:00
MHDA- 2009/07/16 09:00
CRDT- 2009/05/16 09:00
PHST- 2009/05/16 09:00 [entrez]
PHST- 2009/05/16 09:00 [pubmed]
PHST- 2009/07/16 09:00 [medline]
AID - 17/3/198 [pii]
AID - 10.1177/1066896909333415 [doi]
PST - ppublish
SO  - Int J Surg Pathol. 2009 Jun;17(3):198-205. doi: 10.1177/1066896909333415.