PMID- 19445118
OWN - NLM
STAT- MEDLINE
DCOM- 20090904
LR  - 20090518
IS  - 1001-4454 (Print)
IS  - 1001-4454 (Linking)
VI  - 32
IP  - 1
DP  - 2009 Jan
TI  - [Study on the fingerprint of processed Rhizoma Atractylodis Macrocephalae by 
      HPLC].
PG  - 35-8
AB  - OBJECTIVE: To establish HPLC fingerprint for the quality control of processed 
      Rhizoma Atractylodis Macrocephalae (PRAM). METHODS: 14 batches of PRAM were 
      collected from different places and were analyzed with the developed HPLC 
      fingerprints method. The HPLC separation was performed on a Kromasil C18 
      analytical column (250 mm x 4.6 mm, 5 microm), and gradient elution was performed 
      by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min 
      and the detection wavelength was at 242 nm. The temperature of column was 25 
      degrees C. RESULTS: Sixteen mutual peaks were selected in chromatography. Among 
      the obtained fingerprints, the most of the detected peaks were separated 
      effectively. The methodological evaluation showed that the method had a good 
      repeatability. CONCLUSION: The RSD of relative retention time of mutual peaks 
      which existed in all samples was less than 1.1%. The results of peak areas were 
      in accordance with the request of fingerprint. The established fingerprint can be 
      used for the quality control and species identifying of PRAM.
FAU - Chen, Qin-hua
AU  - Chen QH
AD  - Department of Pharmacy, Affiliated Dongfeng Hospital, Yunyang Medical College, 
      Shiyan 442008, China. cqh77@163.com
FAU - Li, Peng
AU  - Li P
FAU - He, Jing
AU  - He J
FAU - Zhu, Jun
AU  - Zhu J
FAU - Chen, Shu-yan
AU  - Chen SY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhong Yao Cai
JT  - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
JID - 9426370
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Asteraceae/*chemistry
MH  - *Chromatography, High Pressure Liquid
MH  - Drugs, Chinese Herbal/chemistry
MH  - Plants, Medicinal/*chemistry
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Rhizome/chemistry
EDAT- 2009/05/19 09:00
MHDA- 2009/09/05 06:00
CRDT- 2009/05/19 09:00
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/09/05 06:00 [medline]
PST - ppublish
SO  - Zhong Yao Cai. 2009 Jan;32(1):35-8.