PMID- 19445231
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100526
LR  - 20090518
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 29
IP  - 2
DP  - 2009 Feb
TI  - [Study on the energy transfer fluorescence quenching reaction of acridine 
      orange-4-(2-pyridylazo) resorcinol-V(V) system and analytical application].
PG  - 476-8
AB  - An energy transfer technique for 4-(2-pyridylazo) resorcinol (PAR)-vanadium(V) 
      and acridine orange (AO) was studied, and the optimum conditions of energy 
      transfer system were also experimented. It was found that in citrate-Na2 HPO4 
      buffer solution at pH = 5.5, energy transfers from AO to vanadium(V)-PAR 
      complexes. A new method based on energy transfer fluorescence quenching for the 
      determination of trace vanadium(V) with AO-PAR-V(V) was established. The equation 
      of linear regression is deltaF = 165.4c+2.5, and the determination range of 
      vanadium is 0.012-0.5 microg x mL(-1), with detection limit of 0.004 5 microg x 
      mL(-1). The correlation coefficient is R = 0.998 5, and relative standard 
      deviation is 0.6%. The fluorescence reaction is completed within 15 minutes, and 
      relative fluorescence intensity remains unchanged for 2.5 hours. The influence of 
      foreign ions on the determination of V (0.5 microg x mL(-1)) was examined, with a 
      related error of 5%. The method has been used in the determination of trace 
      vanadium in biological samples with the relative error of 6.98%, which meets the 
      requirements of trace analysis.
FAU - Hou, Ming
AU  - Hou M
AD  - Material and Chemistry Department, Guilin University of Technology, Guilin 
      541004, China. glhou@glite.edu.cn
FAU - Feng, Li-E
AU  - Feng LE
FAU - Jiang, Zhi-Liang
AU  - Jiang ZL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
EDAT- 2009/05/19 09:00
MHDA- 2009/05/19 09:01
CRDT- 2009/05/19 09:00
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/05/19 09:01 [medline]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Feb;29(2):476-8.