PMID- 19445992
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20090811
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 70
IP  - 8
DP  - 2009 Aug
TI  - Back to the future: application of contemporary technology to long-standing 
      questions about the clinical relevance of human leukocyte antigen-specific 
      alloantibodies in renal transplantation.
PG  - 563-8
LID - 10.1016/j.humimm.2009.05.001 [doi]
AB  - Luminex technology allows the accurate identification of human leukocyte antigen 
      (HLA) class I and class II-specific antibodies at levels below the threshold 
      detectable by either conventional complement-dependent lymphocytotoxicity or flow 
      cytometry. The technology enables the analysis of complex antibody profiles in 
      sensitized patients and gives improved definition of acceptable and unacceptable 
      HLA specificities to guide donor kidney allocation. This helps to facilitate 
      virtual cross-matching and avoid inappropriate shipping of kidneys for 
      incompatible patients in distant centers. Luminex allows the cause of a positive 
      cross-match test to be determined in a clinically relevant time scale and, when 
      used in conjunction with lymphocytotoxic and flow cytometric cross-matching, it 
      provides an assessment of the level of immunological risk in patients being 
      considered as potential recipients for a particular donor kidney. Information is 
      now emerging to enable the full clinical potential of Luminex to be realized.
FAU - Taylor, Craig J
AU  - Taylor CJ
AD  - Tissue Typing Laboratory, Cambridge University Hospitals NHS Foundation Trust, 
      Addenbrooke's Hospital, Hills Road, Cambridge, UK. 
      craig.taylor@addenbrookes.nhs.uk
FAU - Kosmoliaptsis, Vasilis
AU  - Kosmoliaptsis V
FAU - Summers, Dominic M
AU  - Summers DM
FAU - Bradley, J Andrew
AU  - Bradley JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090513
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Complement System Proteins/metabolism
MH  - Cytotoxicity Tests, Immunologic
MH  - Flow Cytometry
MH  - Graft Rejection/blood/*diagnosis/*immunology/prevention & control
MH  - HLA Antigens/*immunology
MH  - Histocompatibility
MH  - Humans
MH  - Immunosorbent Techniques
MH  - Isoantibodies/*blood/immunology
MH  - *Kidney Transplantation
MH  - Microspheres
MH  - Organ Preservation
MH  - Predictive Value of Tests
MH  - Risk Factors
MH  - Sensitivity and Specificity
EDAT- 2009/05/19 09:00
MHDA- 2009/10/10 06:00
CRDT- 2009/05/19 09:00
PHST- 2009/03/09 00:00 [received]
PHST- 2009/04/30 00:00 [revised]
PHST- 2009/05/07 00:00 [accepted]
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
AID - S0198-8859(09)00121-9 [pii]
AID - 10.1016/j.humimm.2009.05.001 [doi]
PST - ppublish
SO  - Hum Immunol. 2009 Aug;70(8):563-8. doi: 10.1016/j.humimm.2009.05.001. Epub 2009 
      May 13.