PMID- 19446767
OWN - NLM
STAT- MEDLINE
DCOM- 20090804
LR  - 20211020
IS  - 1873-3476 (Electronic)
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 374
IP  - 1-2
DP  - 2009 Jun 5
TI  - Enhanced cytotoxicity of TATp-bearing paclitaxel-loaded micelles in vitro and in 
      vivo.
PG  - 114-8
LID - 10.1016/j.ijpharm.2009.02.022 [doi]
AB  - Cell-penetrating peptide (TATp) was attached to the distal tips of 
      polyethyleneglycol (PEG) moieties of polyethyleneglycol-phosphatidylethanolamine 
      (PEG-PE) micelles loaded with paclitaxel (PCT). The TATp-modified micelles 
      demonstrated an increased interaction with cancer cells compared to non-modified 
      micelles resulting in a significant increase of the in vitro cytotoxicity to 
      different cancer cells. TATp-modified PCT-loaded micelles were administered 
      intratumorally in mice and the induction of apoptosis in tumor cells was studied 
      after 48h with the Terminal Deoxynucleotidyl Transferase Biotin-dUTP Nick End 
      Labeling (TUNEL) assay using free PCT and TATp-free PCT-loaded PEG-PE micelles as 
      controls. A significant apoptotic cell death was observed in tumors treated with 
      PCT-loaded micelles modified with TATp, while the treatment with free PCT or with 
      non-modified PCT-loaded micelles resulted in much smaller number of 
      TUNEL-positive cells within tumors.
FAU - Sawant, Rupa R
AU  - Sawant RR
AD  - Department of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology 
      and Nanomedicine, Northeastern University, 360 Huntington Avenue, Boston, MA 
      02115, USA.
FAU - Torchilin, Vladimir P
AU  - Torchilin VP
LA  - eng
GR  - R01 EB001961/EB/NIBIB NIH HHS/United States
GR  - R01 EB 001961/EB/NIBIB NIH HHS/United States
GR  - R01 CA121838/CA/NCI NIH HHS/United States
GR  - R01 EB001961-09/EB/NIBIB NIH HHS/United States
GR  - R01 CA 121838/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090327
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Drug Carriers)
RN  - 0 (Gene Products, tat)
RN  - 0 (Micelles)
RN  - 0 (Phosphatidylethanolamines)
RN  - 39382-08-6 (phosphatidylethanolamine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - P88XT4IS4D (Paclitaxel)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*administration & dosage
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Drug Carriers/chemistry
MH  - Female
MH  - Gene Products, tat/*chemistry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Mammary Neoplasms, Experimental/*drug therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Micelles
MH  - Paclitaxel/*administration & dosage
MH  - Phosphatidylethanolamines/chemistry
MH  - Polyethylene Glycols/chemistry
PMC - PMC2684523
MID - NIHMS105835
EDAT- 2009/05/19 09:00
MHDA- 2009/08/06 09:00
PMCR- 2010/06/05
CRDT- 2009/05/19 09:00
PHST- 2009/01/28 00:00 [received]
PHST- 2009/02/28 00:00 [accepted]
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/08/06 09:00 [medline]
PHST- 2010/06/05 00:00 [pmc-release]
AID - S0378-5173(09)00128-8 [pii]
AID - 10.1016/j.ijpharm.2009.02.022 [doi]
PST - ppublish
SO  - Int J Pharm. 2009 Jun 5;374(1-2):114-8. doi: 10.1016/j.ijpharm.2009.02.022. Epub 
      2009 Mar 27.