PMID- 19447190
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20090714
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 154
IP  - 1
DP  - 2009 Sep
TI  - Isolation and cDNA cloning of an antibacterial L-amino acid oxidase from the skin 
      mucus of the great sculpin Myoxocephalus polyacanthocephalus.
PG  - 55-61
LID - 10.1016/j.cbpb.2009.05.006 [doi]
AB  - The skin mucus of the great sculpin Myoxocephalus polyacanthocephalus showed both 
      antibacterial and L-amino acid oxidase (LAO) activities. Antibacterial LAOs were 
      purified from the skin mucus of the M. polyacanthocephalus by column 
      chromatography and named MPLAO1, MPLAO2, and MPLAO3, based on the order of 
      elution by ion-exchange high performance liquid chromatography. cDNA cloning of 
      MPLAO3 revealed that the full-length cDNAwas 2659 bp and encoded the signal 
      peptide (Met1-Ala26) and the mature protein (Val28-Phe520). A homology search 
      using the BLAST program revealed that MPLAO3 shared sequence identity with LAO 
      family proteins, and had 74% identity with the antibacterial LAO from the skin 
      mucus of the rockfish Sebastes schlegeli. MPLAO3 catalyzed the oxidation of only 
      L-lysine with a Km of 0.16 mM. MPLAO3 exhibited potent antibacterial activity 
      against both Gram-positive and Gramnegative bacteria, and was most active against 
      Aeromonas salmonicida JCM7874 with a minimum inhibitory concentration of 0.02 
      microg/mL. The antibacterial activity was attributable to H2O2, because the 
      activity was completely lost in the presence of catalase. The antibacterial LAOs 
      may be involved in the innate immunity of the great sculpin M. 
      polyacanthocephalus skin.
FAU - Nagashima, Yuji
AU  - Nagashima Y
AD  - Department of Food Science and Technology, Tokyo University of Marine Science and 
      Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan. yujicd@kaiyodai.ac.jp
FAU - Tsukamoto, Chihiro
AU  - Tsukamoto C
FAU - Kitani, Yoichiro
AU  - Kitani Y
FAU - Ishizaki, Shoichiro
AU  - Ishizaki S
FAU - Nagai, Hiroshi
AU  - Nagai H
FAU - Yanagimoto, Takashi
AU  - Yanagimoto T
LA  - eng
PT  - Journal Article
DEP - 20090515
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Complementary)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents/*isolation & purification/metabolism/pharmacology
MH  - Bacteria/drug effects
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Fishes/*genetics
MH  - L-Amino Acid Oxidase/*genetics/*isolation & purification/metabolism/pharmacology
MH  - Molecular Sequence Data
MH  - Mucus/*enzymology
MH  - Sequence Alignment
MH  - *Skin
EDAT- 2009/05/19 09:00
MHDA- 2009/09/18 06:00
CRDT- 2009/05/19 09:00
PHST- 2009/03/15 00:00 [received]
PHST- 2009/04/30 00:00 [revised]
PHST- 2009/05/02 00:00 [accepted]
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
AID - S1096-4959(09)00126-2 [pii]
AID - 10.1016/j.cbpb.2009.05.006 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2009 Sep;154(1):55-61. doi: 
      10.1016/j.cbpb.2009.05.006. Epub 2009 May 15.