PMID- 19448629
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20220129
IS  - 1546-1726 (Electronic)
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 12
IP  - 6
DP  - 2009 Jun
TI  - Synaptotagmin-IV modulates synaptic function and long-term potentiation by 
      regulating BDNF release.
PG  - 767-76
LID - 10.1038/nn.2315 [doi]
AB  - Synaptotagmin-IV (syt-IV) is a membrane trafficking protein that influences 
      learning and memory, but its localization and role in synaptic function remain 
      unclear. We found that syt-IV localized to brain-derived neurotrophic factor 
      (BDNF)-containing vesicles in hippocampal neurons. Syt-IV/BDNF-harboring vesicles 
      underwent exocytosis in both axons and dendrites, and syt-IV inhibited BDNF 
      release at both sites. Knockout of syt-IV increased, and overexpression 
      decreased, the rate of synaptic vesicle exocytosis from presynaptic terminals 
      indirectly via changes in postsynaptic release of BDNF. Thus, postsynaptic syt-IV 
      regulates the trans-synaptic action of BDNF to control presynaptic vesicle 
      dynamics. Furthermore, selective loss of presynaptic syt-IV increased spontaneous 
      quantal release, whereas a loss of postsynaptic syt-IV increased quantal 
      amplitude. Finally, syt-IV knockout mice showed enhanced long-term potentiation 
      (LTP), which depended entirely on disinhibition of BDNF release. Thus, regulation 
      of BDNF secretion by syt-IV emerges as a mechanism for maintaining synaptic 
      strength in a useful range during LTP.
FAU - Dean, Camin
AU  - Dean C
AD  - Department of Physiology, University of Wisconsin, Madison, Wisconsin, USA.
FAU - Liu, Huisheng
AU  - Liu H
FAU - Dunning, F Mark
AU  - Dunning FM
FAU - Chang, Payne Y
AU  - Chang PY
FAU - Jackson, Meyer B
AU  - Jackson MB
FAU - Chapman, Edwin R
AU  - Chapman ER
LA  - eng
GR  - NS44057/NS/NINDS NIH HHS/United States
GR  - NS049748/NS/NINDS NIH HHS/United States
GR  - F32 NS049748/NS/NINDS NIH HHS/United States
GR  - MH61876/MH/NIMH NIH HHS/United States
GR  - GM 56827/GM/NIGMS NIH HHS/United States
GR  - R01 NS044057/NS/NINDS NIH HHS/United States
GR  - NS30016/NS/NINDS NIH HHS/United States
GR  - R37 NS030016/NS/NINDS NIH HHS/United States
GR  - R01 GM056827/GM/NIGMS NIH HHS/United States
GR  - R01 MH061876/MH/NIMH NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01 NS030016/NS/NINDS NIH HHS/United States
GR  - F32 NS049748-01A1/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090517
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotransmitter Agents)
RN  - 134193-27-4 (Synaptotagmins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Dendrites/metabolism/ultrastructure
MH  - Down-Regulation/genetics
MH  - Exocytosis/genetics
MH  - Hippocampus/*metabolism/ultrastructure
MH  - Long-Term Potentiation/*genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/*metabolism/ultrastructure
MH  - Neurotransmitter Agents/metabolism
MH  - Presynaptic Terminals/metabolism/ultrastructure
MH  - Synaptic Potentials/genetics
MH  - Synaptic Transmission/*genetics
MH  - Synaptic Vesicles/metabolism/ultrastructure
MH  - Synaptotagmins/genetics/*metabolism
PMC - PMC2846764
MID - NIHMS104345
EDAT- 2009/05/19 09:00
MHDA- 2009/10/10 06:00
PMCR- 2010/03/29
CRDT- 2009/05/19 09:00
PHST- 2008/12/18 00:00 [received]
PHST- 2009/03/20 00:00 [accepted]
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
PHST- 2010/03/29 00:00 [pmc-release]
AID - nn.2315 [pii]
AID - 10.1038/nn.2315 [doi]
PST - ppublish
SO  - Nat Neurosci. 2009 Jun;12(6):767-76. doi: 10.1038/nn.2315. Epub 2009 May 17.