PMID- 19448902
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20190606
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Linking)
VI  - 42
IP  - 6
DP  - 2009 Jun
TI  - Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells 
      via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling.
PG  - 531-6
LID - S0100-879X2009000600009 [pii]
AB  - Angiotensin II (Ang II) plays a crucial role in the pathogenesis of renal 
      diseases. The objective of the present study was to investigate the possible 
      inflammatory effect of Ang II on glomerular endothelial cells and the underlying 
      mechanism. We isolated and characterized primary cultures of rat glomerular 
      endothelial cells (GECs) and observed that Ang II induced the synthesis of 
      monocyte chemoattractant protein-1 (MCP-1) in GECs as demonstrated by Western 
      blot. Ang II stimulation, at concentrations ranging from 0.1 to 10 microm, of rat 
      GECs induced a rapid increase in the generation of reactive oxygen species as 
      indicated by laser fluoroscopy. The level of p47phox protein, an NAD(P)H oxidase 
      subunit, was also increased by Ang II treatment. These effects of Ang II on GECs 
      were all reduced by diphenyleneiodonium (1.0 microm), an NAD(P)H oxidase 
      inhibitor. Ang II stimulation also promoted the activation of nuclear 
      factor-kappa B (NF-kappaB). Telmisartan (1.0 microm), an AT1 receptor blocker, 
      blocked all the effects of Ang II on rat GECs. These data suggest that the 
      inhibition of NAD(P)H oxidase-dependent NF-kappaB signaling reduces the increase 
      in MCP-1 production by GECs induced by Ang II. This may provide a mechanistic 
      basis for the benefits of selective AT1 blockade in dealing with chronic renal 
      disease.
FAU - Pan, Q
AU  - Pan Q
AD  - Department of Pathology, China Medical University Affiliated Shengjing Hospital, 
      Shenyang, PR China.
FAU - Yang, X-H
AU  - Yang XH
FAU - Cheng, Y-X
AU  - Cheng YX
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Benzimidazoles)
RN  - 0 (Benzoates)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (NF-kappa B)
RN  - 0 (Onium Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 11128-99-7 (Angiotensin II)
RN  - 6HJ411TU98 (diphenyleneiodonium)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - U5SYW473RQ (Telmisartan)
SB  - IM
MH  - Angiotensin II/*pharmacology
MH  - Angiotensin II Type 1 Receptor Blockers/pharmacology
MH  - Animals
MH  - Benzimidazoles/pharmacology
MH  - Benzoates/pharmacology
MH  - Blotting, Western
MH  - Chemokine CCL2/*biosynthesis/drug effects
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Inflammation/metabolism
MH  - Kidney Glomerulus/*cytology
MH  - NADPH Oxidases/*antagonists & inhibitors
MH  - NF-kappa B/*metabolism
MH  - Onium Compounds/pharmacology
MH  - Oxidative Stress/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Telmisartan
EDAT- 2009/05/19 09:00
MHDA- 2010/03/03 06:00
CRDT- 2009/05/19 09:00
PHST- 2008/09/25 00:00 [received]
PHST- 2009/03/16 00:00 [accepted]
PHST- 2009/05/19 09:00 [entrez]
PHST- 2009/05/19 09:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - S0100-879X2009000600009 [pii]
AID - 10.1590/s0100-879x2009000600009 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2009 Jun;42(6):531-6. doi: 10.1590/s0100-879x2009000600009.