PMID- 19451278
OWN - NLM
STAT- MEDLINE
DCOM- 20090622
LR  - 20220309
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 185
IP  - 4
DP  - 2009 May 18
TI  - Pro-BDNF-induced synaptic depression and retraction at developing neuromuscular 
      synapses.
PG  - 727-41
LID - 10.1083/jcb.200811147 [doi]
AB  - Postsynaptic cells generate positive and negative signals that retrogradely 
      modulate presynaptic function. At developing neuromuscular synapses, prolonged 
      stimulation of muscle cells induces sustained synaptic depression. We provide 
      evidence that pro-brain-derived neurotrophic factor (BDNF) is a negative 
      retrograde signal that can be converted into a positive signal by 
      metalloproteases at the synaptic junctions. Application of pro-BDNF induces a 
      dramatic decrease in synaptic efficacy followed by a retraction of presynaptic 
      terminals, and these effects are mediated by presynaptic pan-neurotrophin 
      receptor p75 (p75(NTR)), the pro-BDNF receptor. A brief stimulation of myocytes 
      expressing cleavable or uncleavable pro-BDNF elicits synaptic potentiation or 
      depression, respectively. Extracellular application of metalloprotease 
      inhibitors, which inhibits the cleavage of endogenous pro-BDNF, facilitates the 
      muscle stimulation-induced synaptic depression. Inhibition of presynaptic 
      p75(NTR) or postsynaptic BDNF expression also blocks the activity-dependent 
      synaptic depression and retraction. These results support a model in which 
      postsynaptic secretion of a single molecule, pro-BDNF, may stabilize or eliminate 
      presynaptic terminals depending on its proteolytic conversion at the synapses.
FAU - Yang, Feng
AU  - Yang F
AD  - Section on Neural Development and Plasticity, National Institute of Child Health 
      and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Je, Hyun-Soo
AU  - Je HS
FAU - Ji, Yuanyuan
AU  - Ji Y
FAU - Nagappan, Guhan
AU  - Nagappan G
FAU - Hempstead, Barbara
AU  - Hempstead B
FAU - Lu, Bai
AU  - Lu B
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Precursors)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (brain-derived neurotrophic factor precursor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/*physiology
MH  - Muscle Cells/physiology
MH  - Neuromuscular Junction/*growth & development
MH  - Presynaptic Terminals/*ultrastructure
MH  - Protein Precursors/metabolism/*physiology
MH  - Receptor, Nerve Growth Factor/*physiology
MH  - Synapses
MH  - *Synaptic Transmission
MH  - Xenopus
PMC - PMC2711569
EDAT- 2009/05/20 09:00
MHDA- 2009/06/23 09:00
PMCR- 2009/11/18
CRDT- 2009/05/20 09:00
PHST- 2009/05/20 09:00 [entrez]
PHST- 2009/05/20 09:00 [pubmed]
PHST- 2009/06/23 09:00 [medline]
PHST- 2009/11/18 00:00 [pmc-release]
AID - jcb.200811147 [pii]
AID - 200811147 [pii]
AID - 10.1083/jcb.200811147 [doi]
PST - ppublish
SO  - J Cell Biol. 2009 May 18;185(4):727-41. doi: 10.1083/jcb.200811147.