PMID- 19453053
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20090520
IS  - 0172-6390 (Print)
IS  - 0172-6390 (Linking)
VI  - 56
IP  - 89
DP  - 2009 Jan-Feb
TI  - Differential expression of organic anion transporters in rats subjected to 70% or 
      90% hepatectomy.
PG  - 176-80
AB  - BACKGROUND/AIMS: Differences in hepatic organic anion transporters were compared 
      in rats to identify a causative factor for early stage acute hepatic failure 
      after 70% or 90% hepatectomy (Hx). METHODOLOGY: Male Wistar rats (8 weeks, 
      250-330g) were randomly assigned to one of two groups for 70% Hx or 90% Hx, and 
      sacrificed at 0, 6, 12, 24, 48, or 72 hours after Hx. Posthepatectomy expression 
      of the bile salt export pump (Bsep), multidrug resistance proteins 2 and 3 (Mrp2, 
      Mrp3), sodium-dependent taurocholate co-transporting polypeptide (Ntcp), and 
      organic anion transporting polypeptides 1 and 2 (Oatp1, Oatp2) were analyzed by 
      Northern blotting. Serum liver function tests were also performed. RESULTS: 
      Postoperative survival rates at 72 hours after 70% and 90% Hx were 100% and 50%, 
      respectively. mRNA expression of Bsep and Mrp3 was increased after 90% Hx, while 
      decreased after 70% Hx. These values were significantly greater at 12 and 24 
      hours after 90% Hx than after 70% Hx (p < 0.05). In contrast, Mrp2 expression was 
      downregulated to a half of the preoperative level after 90% Hx, while increased 
      after 70% Hx. mRNA expression of uptake transporters (Ntcp, Oatp1, Oatp2) was 
      decreased after 70% Hx and 90% Hx with a similar extent. Total serum bilirubin 
      and bile acid levels were significantly increased after both hepatectomy 
      procedures with a greater extent after 90% Hx. CONCLUSIONS: Alteration of mRNA 
      expression of Bsep, Mrp2 and Mrp3 may be characteristic behavior in the early 
      stage of acute liver failure.
FAU - Sano, Koichiro
AU  - Sano K
AD  - Department of Surgical Oncology and Regulation of Organ Function, Miyazaki 
      University School of Medicine, Miyazaki, Japan.
FAU - Chijiiwa, Kazuo
AU  - Chijiiwa K
FAU - Kai, Masahiro
AU  - Kai M
FAU - Hidaka, Yoshiaki
AU  - Hidaka Y
FAU - Kanemaru, Mikio
AU  - Kanemaru M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Hepatogastroenterology
JT  - Hepato-gastroenterology
JID - 8007849
RN  - 0 (Organic Anion Transporters)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Hepatectomy/*methods
MH  - Liver Function Tests
MH  - Male
MH  - Organic Anion Transporters/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Survival Rate
EDAT- 2009/05/21 09:00
MHDA- 2009/06/26 09:00
CRDT- 2009/05/21 09:00
PHST- 2009/05/21 09:00 [entrez]
PHST- 2009/05/21 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
PST - ppublish
SO  - Hepatogastroenterology. 2009 Jan-Feb;56(89):176-80.