PMID- 19454242
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20131121
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 387
IP  - 2
DP  - 2009 Apr 15
TI  - Heart-cut capillary electrophoresis for drug analysis in mouse blood with 
      electrochemical detection.
PG  - 171-7
LID - 10.1016/j.ab.2009.01.026 [doi]
AB  - A novel separation method, which interfaced capillary zone electrophoresis (CZE) 
      and micellar electrokinetic capillary chromatography (MEKC), was developed for 
      analysis of weak basic compounds. CZE was used as the first separation, from 
      which the effluent components were transferred and further analyzed by MEKC. As 
      the key to successful integration of CZE and MEKC, a new microhole interface was 
      designed. On-line concentration strategies, namely pH junction and sweeping, were 
      employed to avoid sample zone diffusion at the interface. For evaluation of the 
      feasibility and effectiveness of the capillary electrophoresis (CE) system, basic 
      cardiovascular drugs were used as model compounds. The resulting electropherogram 
      was quite different from that of either CZE or MEKC separation. The relative 
      standard deviations (RSDs) of peak height, peak area, and migration time were in 
      the ranges of 2.7% to 4.5%, 1.3% to 3.6%, and 0.8% to 1.4%, respectively, and 
      detection limits (signal/noise=3) were 0.015 to 0.052 mg/L. The proposed method 
      was successfully applied to the determination of basic cardiovascular drugs in 
      mouse blood.
FAU - Zhang, Zhao-Xiang
AU  - Zhang ZX
AD  - College of Chemistry and Molecular Engineering, Qingdao University of Science and 
      Technology, Qingdao 266042, China. zhangzhx02@ustc.edu
FAU - Zhang, Xiao-Wei
AU  - Zhang XW
FAU - Zhang, Shu-Sheng
AU  - Zhang SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090124
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Pharmaceutical Preparations)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - GEB06NHM23 (Metoprolol)
SB  - IM
MH  - Animals
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Electrochemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Hydrogen-Ion Concentration
MH  - Metoprolol/blood
MH  - Mice
MH  - Pharmaceutical Preparations/*blood
MH  - Propranolol/blood
EDAT- 2009/05/21 09:00
MHDA- 2009/06/24 09:00
CRDT- 2009/05/21 09:00
PHST- 2008/10/11 00:00 [received]
PHST- 2009/01/20 00:00 [revised]
PHST- 2009/01/21 00:00 [accepted]
PHST- 2009/05/21 09:00 [entrez]
PHST- 2009/05/21 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
AID - S0003-2697(09)00059-1 [pii]
AID - 10.1016/j.ab.2009.01.026 [doi]
PST - ppublish
SO  - Anal Biochem. 2009 Apr 15;387(2):171-7. doi: 10.1016/j.ab.2009.01.026. Epub 2009 
      Jan 24.