PMID- 19456133
OWN - NLM
STAT- MEDLINE
DCOM- 20090910
LR  - 20121115
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 12
DP  - 2009 Jun 24
TI  - Beta-sitosterol inhibits cell growth and induces apoptosis in SGC-7901 human 
      stomach cancer cells.
PG  - 5211-8
LID - 10.1021/jf803878n [doi]
AB  - Beta-sitosterol is an important phytosterol found in plant food. It has been 
      shown to have antiproliferative effects on cancers of the colon, breast, and 
      prostate, but its effect on stomach cancer cells in vitro is unknown. 
      Proliferation, cytotoxicity, and apoptosis in SGC-7901 human stomach cancer cells 
      were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 
      (MTT) assay, clone formation, lactate dehydrogenase (LDH) leakage assay, acridine 
      orange (AO)/ethidium bromide (EB) double staining, 4',6-diamidine-2'-phenylindole 
      dihydrochloride (DAPI) staining, comet assay, and Western blotting. The results 
      showed that beta-sitosterol suppresses the proliferation and induces the cell 
      cytotoxicity of SGC-7901 stomach cancer cells in a time- and dose-dependent 
      manner. Cells treated with different concentrations of beta-sitosterol also 
      showed changes typical of apoptosis: morphological changes, DNA damage, increased 
      expression of pro-caspase-3 and bax (p < 0.05), and activation of pro-caspase-3 
      and suppression of bcl-2 expression (p < 0.05). This study therefore revealed 
      that beta-sitosterol significantly inhibits the growth and induces the apoptosis 
      of SGC-7901 human stomach cancer cells in vitro. The decrease of the bcl-2/bax 
      ratio and DNA damage may be the critical mechanisms of apoptosis induced by 
      beta-sitosterol in SGC-7901 human stomach cancer cells.
FAU - Zhao, Yonghuan
AU  - Zhao Y
AD  - Department of Nutrition and Food Hygiene, Public Health College of Harbin Medical 
      University, Harbin, China.
FAU - Chang, Sam K C
AU  - Chang SK
FAU - Qu, Gang
AU  - Qu G
FAU - Li, Tianwen
AU  - Li T
FAU - Cui, Hongbin
AU  - Cui H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Sitosterols)
RN  - 5LI01C78DD (gamma-sitosterol)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - *Down-Regulation
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Sitosterols/*pharmacology
MH  - Stomach Neoplasms/*drug therapy/physiopathology
EDAT- 2009/05/22 09:00
MHDA- 2009/09/11 06:00
CRDT- 2009/05/22 09:00
PHST- 2009/05/22 09:00 [entrez]
PHST- 2009/05/22 09:00 [pubmed]
PHST- 2009/09/11 06:00 [medline]
AID - 10.1021/jf803878n [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Jun 24;57(12):5211-8. doi: 10.1021/jf803878n.