PMID- 19456762
OWN - NLM
STAT- MEDLINE
DCOM- 20100818
LR  - 20100108
IS  - 1365-2230 (Electronic)
IS  - 0307-6938 (Linking)
VI  - 34
IP  - 8
DP  - 2009 Dec
TI  - Identification of granzyme B-expressing CD-8-positive T cells in lymphocytic 
      inflammatory infiltrate in cutaneous lupus erythematosus and in dermatomyositis.
PG  - 910-4
LID - 10.1111/j.1365-2230.2009.03297.x [doi]
AB  - BACKGROUND: Lupus erythematosus (LE) and dermatomyositis (DM) are interface 
      dermatitis, histologically characterized by formation of colloid bodies and a 
      CD4+ CD8+ lymphocyte infiltrate. Colloid bodies are examples of intraepidermal 
      apoptosis. Granzyme (Gr)B, synthesized by activated cytotoxic lymphocytes, is a 
      serine protease able to prime apoptosis. GrB+ lymphocytes have been previously 
      found in infiltrates in skin lesions from other types of interface dermatitis. 
      AIM: To evaluate, on histological skin specimens from patients with LE and DM, 
      GrB expression as a mediator of keratinocyte apoptosis in lymphocyte infiltrate. 
      METHODS: In total, 22 patients with cutaneous LE [9 with discoid lupus 
      erythematosus (DLE), 9 with subacute lupus erythematosus (SCLE) and 4 with 
      systemic lupus erythematosus (SLE)] and 20 patients with DM were studied. Skin 
      specimens underwent immunohistochemical staining with antibodies to CD3, CD4, CD8 
      and GrB. RESULTS: Immunohistochemical study with GrB was positive in 17 of the 22 
      patients with LE but only 2 of the 20 patients with DM. In LE, in systemic and 
      subacute forms of LE, the median obtained was < 10 (+) whereas in the chronic 
      forms, the median was 10-50% (++). Patients with DM were negative for GrB. 
      CONCLUSIONS: In LE, a correlation between GrB+ lymphocyte and the presence of DLE 
      form was found, but in DM, GrB is poorly expressed. GrB labels a subpopulation of 
      effector cells involved in ongoing cytotoxic action and should be considered as a 
      specific marker showing the extent of the direct local cytotoxic damage. GrB 
      could play a role in the induction of skin apoptosis mechanisms in LE.
FAU - Grassi, M
AU  - Grassi M
AD  - Dermatology Clinic II, University of Turin, Turin, Italy. marcellagrassi@yahoo.it
FAU - Capello, F
AU  - Capello F
FAU - Bertolino, L
AU  - Bertolino L
FAU - Seia, Z
AU  - Seia Z
FAU - Pippione, M
AU  - Pippione M
LA  - eng
PT  - Journal Article
DEP - 20090518
PL  - England
TA  - Clin Exp Dermatol
JT  - Clinical and experimental dermatology
JID - 7606847
RN  - EC 3.4.21.- (Granzymes)
SB  - IM
CIN - Clin Exp Dermatol. 2010 Aug;35(6):669-70. PMID: 20345990
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Apoptosis/genetics
MH  - CD8-Positive T-Lymphocytes/*metabolism
MH  - Dermatomyositis/immunology/*metabolism
MH  - Female
MH  - Granzymes/*metabolism
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/immunology/*metabolism
MH  - Male
MH  - Middle Aged
EDAT- 2009/05/22 09:00
MHDA- 2010/08/19 06:00
CRDT- 2009/05/22 09:00
PHST- 2009/05/22 09:00 [entrez]
PHST- 2009/05/22 09:00 [pubmed]
PHST- 2010/08/19 06:00 [medline]
AID - CED3297 [pii]
AID - 10.1111/j.1365-2230.2009.03297.x [doi]
PST - ppublish
SO  - Clin Exp Dermatol. 2009 Dec;34(8):910-4. doi: 10.1111/j.1365-2230.2009.03297.x. 
      Epub 2009 May 18.