PMID- 19457432
OWN - NLM
STAT- MEDLINE
DCOM- 20090910
LR  - 20211028
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 391
IP  - 2
DP  - 2009 Aug 15
TI  - Ratiometric Raman spectroscopy for quantification of protein oxidative damage.
PG  - 121-6
LID - 10.1016/j.ab.2009.05.019 [doi]
AB  - A novel ratiometric Raman spectroscopic (RMRS) method has been developed for 
      quantitative determination of protein carbonyl levels. Oxidized bovine serum 
      albumin (BSA) and oxidized lysozyme were used as model proteins to demonstrate 
      this method. The technique involves conjugation of protein carbonyls with 
      dinitrophenyl hydrazine (DNPH), followed by drop coating deposition Raman 
      spectral acquisition (DCDR). The RMRS method is easy to implement because it 
      requires only one conjugation reaction, uses a single spectral acquisition, and 
      does not require sample calibration. Characteristic peaks from both protein and 
      DNPH moieties are obtained in a single spectral acquisition, allowing the protein 
      carbonyl level to be calculated from the peak intensity ratio. Detection 
      sensitivity for the RMRS method is approximately 0.33 pmol carbonyl per 
      measurement. Fluorescence and/or immunoassay-based techniques only detect a 
      signal from the labeling molecule and, thus, yield no structural or quantitative 
      information for the modified protein, whereas the RMRS technique allows protein 
      identification and protein carbonyl quantification in a single experiment.
FAU - Zhang, Dongmao
AU  - Zhang D
AD  - Department of Chemistry, Mississippi State University, Mississippi State, MS 
      39762, USA. dz33@msstate.edu
FAU - Jiang, Dongping
AU  - Jiang D
FAU - Yanney, Michael
AU  - Yanney M
FAU - Zou, Sige
AU  - Zou S
FAU - Sygula, Andrzej
AU  - Sygula A
LA  - eng
GR  - ZIA AG000366-02/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
DEP - 20090518
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Hydrazines)
RN  - 0 (dinitrophenylhydrazine)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Hydrazines/chemistry
MH  - Muramidase/*analysis/chemistry
MH  - Oxidation-Reduction
MH  - *Oxidative Stress
MH  - Serum Albumin, Bovine/*analysis/chemistry
MH  - Spectrum Analysis, Raman/*methods
PMC - PMC2773654
MID - NIHMS155500
EDAT- 2009/05/22 09:00
MHDA- 2009/09/11 06:00
PMCR- 2009/11/05
CRDT- 2009/05/22 09:00
PHST- 2009/03/27 00:00 [received]
PHST- 2009/05/06 00:00 [revised]
PHST- 2009/05/12 00:00 [accepted]
PHST- 2009/05/22 09:00 [entrez]
PHST- 2009/05/22 09:00 [pubmed]
PHST- 2009/09/11 06:00 [medline]
PHST- 2009/11/05 00:00 [pmc-release]
AID - S0003-2697(09)00334-0 [pii]
AID - 10.1016/j.ab.2009.05.019 [doi]
PST - ppublish
SO  - Anal Biochem. 2009 Aug 15;391(2):121-6. doi: 10.1016/j.ab.2009.05.019. Epub 2009 
      May 18.