PMID- 19460723
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20161125
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 29
IP  - 5
DP  - 2009 May
TI  - [Effect of the proteasome inhibitor MG-132 on hyperoxic lung injury and its 
      mechanism in rats].
PG  - 970-3, 978
AB  - OBJECTIVE: To observe the effects of proteasome inhibitor MG-132 on hyperoxic 
      lung injury in rats and explore the mechanism. METHODS: Thirty SD rats were 
      randomly divided into 3 groups, namely the normoxic group, hyperoxic group, and 
      hyperoxic with MG-132 treatment group, and rat models of hyperoxic 
      exposure-induced lung injury were established in the latter two groups. After 
      pathological grading of the lung injury under optical microscope and 
      determination of the wet/dry weight ratio of the lung tissue, the expressions of 
      ubiquitin protein and nuclear factor-kappaB (NF-kappaB) p56 and the activity of 
      proteasome 20S and myeloperoxidase (MPO) were detected. Tumor necrosis 
      factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expressions in the lung tissue 
      were also detected. RESULTS: The rats with hyperoxic exposure showed obvious 
      pulmonary edema and increased wet/dry weight ratio of the lung tissue (P<0.01), 
      which were significantly alleviated with MG-132 treatment (P<0.01). Compared with 
      the normoxic group, hyperoxic exposure resulted in significant lung pathologies 
      (P<0.01), which was reduced after MG-132 treatment. Immunohistochemistry and 
      Western blotting demonstrated increased expression of ubiquitin protein in the 
      lung tissue after hyperoxic exposure (P<0.01), which was lowered by MG-132 
      treatment (P<0.01). Proteasome 20S activity was obviously enhanced in the 
      hyperoxic group (P<0.01) but lowered by MG-132 treatment (P<0.01). Hyperoxic 
      exposure also caused obviously enhanced MPO activity and expressions of 
      NF-kappaB, TNF-alpha, and IL-6 (P<0.01), which were all reduced by MG-132 
      treatment (P<0.05). CONCLUSION: MG-132 alleviates hyperoxic lung injury probably 
      by inhibiting the NF-kappaB/inflammatory factor pathways.
FAU - Huang, Yu-ge
AU  - Huang YG
AD  - Graduate College of Southern Medical Unversity, Guangzhou 510515, China. 
      kenbobozj@yahoo.com.cn
FAU - Feng, Zhi-chun
AU  - Feng ZC
FAU - Yu, Yan-liang
AU  - Yu YL
FAU - Xiao, Fang-fang
AU  - Xiao FF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Interleukin-6)
RN  - 0 (Leupeptins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Ubiquitin)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cysteine Proteinase Inhibitors/*pharmacology
MH  - Female
MH  - Hyperoxia/*complications
MH  - Interleukin-6/metabolism
MH  - Leupeptins/*pharmacology
MH  - Lung Injury/etiology/metabolism/*pathology
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - Peroxidase/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Ubiquitin/metabolism
EDAT- 2009/05/23 09:00
MHDA- 2010/05/05 06:00
CRDT- 2009/05/23 09:00
PHST- 2009/05/23 09:00 [entrez]
PHST- 2009/05/23 09:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2009 May;29(5):970-3, 978.