PMID- 19461207
OWN - NLM
STAT- MEDLINE
DCOM- 20090820
LR  - 20211020
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 10
IP  - 2
DP  - 2009 Jun
TI  - Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory 
      syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or 
      non-vaccinating farms.
PG  - 121-30
AB  - The 23 open reading frame (ORF) 5 sequences of Korean type II porcine 
      reproductive and respiratory syndrome virus (PRRSV) were collected from viremic 
      sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms 
      from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 
      sequences, including type I Korean PRRSV, and previously reported or collected 
      sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was 
      newly identified in the viremic sera collected from 2007 to 2008. And of the type 
      I PRRSVs, one subgroup had 87.2 approximately 88.9% similarity with the Lelystad 
      virus, showing a close relationship with the 27 approximately 2003 strain of 
      Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that 
      they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently 
      collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell 
      epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably 
      different from the MLV in subgroups 3 and 4. In conclusion, the existence of type 
      I PRRSV, which was genetically different from Lelystad virus (Prototype of type I 
      PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the 
      MLV-vaccinating farms indicated the need for new vaccine approaches for the 
      control of PRRSV in Korea.
FAU - Kim, Hye Kwon
AU  - Kim HK
AD  - Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine 
      and BK21 Program for Veterinary Science, Seoul National University, Seoul, Korea.
FAU - Yang, Jeong Sun
AU  - Yang JS
FAU - Moon, Hyoung Joon
AU  - Moon HJ
FAU - Park, Seong Jun
AU  - Park SJ
FAU - Luo, Yuzi
AU  - Luo Y
FAU - Lee, Chul Seung
AU  - Lee CS
FAU - Song, Dae Sub
AU  - Song DS
FAU - Kang, Bo Kyu
AU  - Kang BK
FAU - Ann, Soo Kyung
AU  - Ann SK
FAU - Jun, Chan Hyuk
AU  - Jun CH
FAU - Park, Bong Kyun
AU  - Park BK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Evolution, Molecular
MH  - Korea
MH  - *Open Reading Frames
MH  - Phylogeny
MH  - Pilot Projects
MH  - Porcine Reproductive and Respiratory Syndrome/blood/genetics/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Viral Vaccines/immunology/standards
MH  - Viremia/genetics/immunology/virology
PMC - PMC2801115
EDAT- 2009/05/23 09:00
MHDA- 2009/08/21 09:00
PMCR- 2009/06/01
CRDT- 2009/05/23 09:00
PHST- 2009/05/23 09:00 [entrez]
PHST- 2009/05/23 09:00 [pubmed]
PHST- 2009/08/21 09:00 [medline]
PHST- 2009/06/01 00:00 [pmc-release]
AID - 200906121 [pii]
AID - 10.4142/jvs.2009.10.2.121 [doi]
PST - ppublish
SO  - J Vet Sci. 2009 Jun;10(2):121-30. doi: 10.4142/jvs.2009.10.2.121.