PMID- 19462206
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20211020
IS  - 1573-1561 (Electronic)
IS  - 0098-0331 (Linking)
VI  - 35
IP  - 7
DP  - 2009 Jul
TI  - Natural ligands of porcine olfactory binding proteins.
PG  - 741-51
LID - 10.1007/s10886-009-9645-1 [doi]
AB  - Knowledge of endogenous ligands of olfactory binding proteins is a prerequisite 
      for studying their role in odor and pheromone transduction. Here, we report the 
      extraction, derivatization, and characterization by gas chromatography-mass 
      spectrometry of the natural ligands of pig, Sus scrofa (L.), Von Ebner's Gland 
      protein (VEG) and odorant binding protein (OBP). We identified two isoforms (VEG1 
      and VEG2), which differed only by the linkage of an O-N-acetylglucosamine 
      (O-GlcNac) group on VEG1. The natural ligands of VEG1 were characterized as two 
      isomers of testosterone, whereas ligands of VEG2 and OBP were fatty acids or 
      their derivatives. Our findings suggest that the binding specificity of VEG1 for 
      steroids is governed by the presence of an O-GlcNac moiety on the protein. This 
      specificity was confirmed by the binding of radiolabeled testosterone only by 
      VEG1 in an in-gel binding assay. This is the first evidence for a 
      post-translational modification in the process of odorant discrimination by 
      olfactory binding proteins.
FAU - Le Danvic, Chrystelle
AU  - Le Danvic C
AD  - INRA, UMR 8576 CNRS/Universite Lille1, Unite de Glycobiologie Structurale et 
      Fonctionnelle, 59655, Villeneuve d'Ascq Cedex, France.
FAU - Guiraudie-Capraz, Gaelle
AU  - Guiraudie-Capraz G
FAU - Abderrahmani, Djamel
AU  - Abderrahmani D
FAU - Zanetta, Jean-Pierre
AU  - Zanetta JP
FAU - Nagnan-Le Meillour, Patricia
AU  - Nagnan-Le Meillour P
LA  - eng
PT  - Journal Article
DEP - 20090522
PL  - United States
TA  - J Chem Ecol
JT  - Journal of chemical ecology
JID - 7505563
RN  - 0 (Ligands)
RN  - 0 (Lipocalin 1)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Odorant)
RN  - 0 (odorant-binding protein)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 3XMK78S47O (Testosterone)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/chemistry
MH  - Animals
MH  - Gas Chromatography-Mass Spectrometry
MH  - Ligands
MH  - Lipocalin 1/*chemistry
MH  - Palmitic Acid/chemistry
MH  - Protein Isoforms/chemistry
MH  - Receptors, Odorant/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Sus scrofa
MH  - Testosterone/pharmacology
EDAT- 2009/05/23 09:00
MHDA- 2009/10/01 06:00
CRDT- 2009/05/23 09:00
PHST- 2009/02/20 00:00 [received]
PHST- 2009/05/15 00:00 [accepted]
PHST- 2009/05/08 00:00 [revised]
PHST- 2009/05/23 09:00 [entrez]
PHST- 2009/05/23 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
AID - 10.1007/s10886-009-9645-1 [doi]
PST - ppublish
SO  - J Chem Ecol. 2009 Jul;35(7):741-51. doi: 10.1007/s10886-009-9645-1. Epub 2009 May 
      22.