PMID- 19464739
OWN - NLM
STAT- MEDLINE
DCOM- 20090811
LR  - 20181201
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 160
IP  - 1-2
DP  - 2009 Sep
TI  - Development of a vaccine vector based on a subgenomic replicon of porcine 
      reproductive and respiratory syndrome virus.
PG  - 22-8
LID - 10.1016/j.jviromet.2009.04.004 [doi]
AB  - In this study, a DNA-launched subgenomic replicon of porcine reproductive and 
      respiratory syndrome virus (PRRSV) was developed for use as a vaccine vector. 
      This replicon plasmid contained a PRRSV subgenome without structural genes 
      ORF2-ORF6, and was under the transcriptional control of the immediate-early 
      promoter of cytomegalovirus (CMV). Using enhanced green fluorescent protein 
      (EGFP) as a reporter gene, the DNA-launched subgenomic replicon of PRRSV, named 
      pOK-Clone20-rep, could express heterologous genes in vitro. After direct 
      inoculation of pOK-Clone20-rep, mice developed antibody responses that were 
      specific for both the EGFP and the N gene in a dose-dependent manner. 
      Furthermore, mice immunized with pOK-Clone20-rep at a dose of 100microg showed 
      significantly enhanced levels of IFN-gamma compared with those inoculated with 
      100microg of pcD-EGFP, a conventional DNA vaccine that encodes EGFP. In summary, 
      the results show that the DNA-launched subgenomic replicon of PRRSV could not 
      only mediate foreign gene expression in vitro but also induced an immune response 
      in vivo. Similarly, expression and immunogenicity of the N gene also strengthened 
      the potential of the replicon to serve as a vaccine vector expressing multiple 
      genes. It therefore provides a useful tool for vaccine development and the study 
      of the transcription and replication of PRRSV.
FAU - Huang, Qinfeng
AU  - Huang Q
AD  - Huazhong Agricultural University, Wuhan, Hubei, China.
FAU - Yao, Qingxia
AU  - Yao Q
FAU - Fan, Huiying
AU  - Fan H
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Si, Youhui
AU  - Si Y
FAU - Chen, Huanchun
AU  - Chen H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090411
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cytomegalovirus/genetics
MH  - Female
MH  - Genes, Reporter
MH  - *Genetic Vectors
MH  - Green Fluorescent Proteins/genetics/immunology
MH  - Interferon-gamma/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plasmids
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - *Replicon
MH  - Vaccines, DNA/genetics/immunology
MH  - Viral Proteins/genetics/immunology
MH  - Viral Vaccines/*genetics/*immunology
EDAT- 2009/05/26 09:00
MHDA- 2009/08/12 09:00
CRDT- 2009/05/26 09:00
PHST- 2008/11/27 00:00 [received]
PHST- 2009/03/30 00:00 [revised]
PHST- 2009/04/02 00:00 [accepted]
PHST- 2009/05/26 09:00 [entrez]
PHST- 2009/05/26 09:00 [pubmed]
PHST- 2009/08/12 09:00 [medline]
AID - S0166-0934(09)00166-9 [pii]
AID - 10.1016/j.jviromet.2009.04.004 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Sep;160(1-2):22-8. doi: 10.1016/j.jviromet.2009.04.004. 
      Epub 2009 Apr 11.