PMID- 19464796
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20211020
IS  - 1872-6623 (Electronic)
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 144
IP  - 3
DP  - 2009 Aug
TI  - Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical 
      hyperalgesia.
PG  - 270-277
LID - 10.1016/j.pain.2009.04.021 [doi]
AB  - The involvement of TRPV1 and TRPA1 in mediating craniofacial muscle nociception 
      and mechanical hyperalgesia was investigated in male Sprague-Dawley rats. First, 
      we confirmed the expression of TRPV1 in masseter afferents in rat trigeminal 
      ganglia (TG), and provided new data that TRPA1 is also expressed in primary 
      afferents innervating masticatory muscles in double-labeling immunohistochemistry 
      experiments. We then examined whether the activation of each TRP channel in the 
      masseter muscle evokes acute nocifensive responses and leads to the development 
      of masseter hypersensitivity to mechanical stimulation using the behavioral 
      models that have been specifically designed and validated for the craniofacial 
      system. Intramuscular injections with specific agonists for TRPV1 and TRPA1, 
      capsaicin and mustard oil (MO), respectively, produced immediate nocifensive 
      hindpaw responses followed by prolonged mechanical hyperalgesia in a 
      concentration-dependent manner. Pretreatment of the muscle with a TRPV1 
      antagonist, capsazepine, effectively attenuated the capsaicin-induced muscle 
      nociception and mechanical hyperalgesia. Similarly, pretreatment of the muscle 
      with a selective TRPA1 antagonist, AP18, significantly blocked the MO-induced 
      muscle nociception and mechanical hyperalgesia. We confirmed these data with 
      another set of selective antagonist for TRPV1 and TRPA1, AMG9810 and HC030031, 
      respectively. Collectively, these results provide compelling evidence that TRPV1 
      and TRPA1 can functionally contribute to muscle nociception and hyperalgesia, and 
      suggest that TRP channels expressed in muscle afferents can engage in the 
      development of pathologic muscle pain conditions.
FAU - Ro, Jin Y
AU  - Ro JY
AD  - University of Maryland Baltimore School of Dentistry, Program in Neuroscience, 
      Department of Neural and Pain Sciences, 650 W. Baltimore Street, Baltimore, MD 
      21201, USA.
FAU - Lee, Jong-Seok
AU  - Lee JS
FAU - Zhang, Youping
AU  - Zhang Y
LA  - eng
GR  - R01 DE016062/DE/NIDCR NIH HHS/United States
GR  - R01 DE016062-04/DE/NIDCR NIH HHS/United States
GR  - P01 DE16062/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090522
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 0 (3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo(b)(1,4)dioxin-6-yl)acrylamide)
RN  - 0 (Acrylamides)
RN  - 0 (Ankyrins)
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Calcium Channels)
RN  - 0 (Plant Oils)
RN  - 0 (Sensory System Agents)
RN  - 0 (TRPA1 Cation Channel)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpa1 protein, rat)
RN  - 0 (Trpv1 protein, rat)
RN  - LFW48MY844 (capsazepine)
RN  - S07O44R1ZM (Capsaicin)
RN  - TYY1MA9BSY (mustard oil)
SB  - IM
MH  - Acrylamides/pharmacology
MH  - Afferent Pathways/drug effects/metabolism
MH  - Animals
MH  - Ankyrins
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - Calcium Channels/drug effects/*metabolism
MH  - Capsaicin/analogs & derivatives/pharmacology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Facial Pain/*metabolism/physiopathology
MH  - Hyperalgesia/*metabolism/physiopathology
MH  - Injections, Intramuscular
MH  - Male
MH  - Masticatory Muscles/*innervation/physiopathology
MH  - Mustard Plant
MH  - Nociceptors/drug effects/*metabolism
MH  - Pain Measurement/methods
MH  - Pain Threshold/drug effects/physiology
MH  - Plant Oils/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory Receptor Cells/drug effects/metabolism
MH  - Sensory System Agents/pharmacology
MH  - TRPA1 Cation Channel
MH  - TRPC Cation Channels
MH  - TRPV Cation Channels/drug effects/*metabolism
MH  - Trigeminal Ganglion/drug effects/metabolism
PMC - PMC2789550
MID - NIHMS133405
COIS- There are no conflicts of interest.
EDAT- 2009/05/26 09:00
MHDA- 2009/10/01 06:00
PMCR- 2010/08/01
CRDT- 2009/05/26 09:00
PHST- 2009/01/08 00:00 [received]
PHST- 2009/03/26 00:00 [revised]
PHST- 2009/04/20 00:00 [accepted]
PHST- 2009/05/26 09:00 [entrez]
PHST- 2009/05/26 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2010/08/01 00:00 [pmc-release]
AID - 00006396-200908000-00011 [pii]
AID - 10.1016/j.pain.2009.04.021 [doi]
PST - ppublish
SO  - Pain. 2009 Aug;144(3):270-277. doi: 10.1016/j.pain.2009.04.021. Epub 2009 May 22.