PMID- 19465512
OWN - NLM
STAT- MEDLINE
DCOM- 20091008
LR  - 20231213
IS  - 1931-3543 (Electronic)
IS  - 0012-3692 (Linking)
VI  - 136
IP  - 3
DP  - 2009 Sep
TI  - Decreased small airway and alveolar CD83+ dendritic cells in COPD.
PG  - 726-733
LID - S0012-3692(09)60540-7 [pii]
LID - 10.1378/chest.08-2824 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) have been reported to be increased in the small 
      airways of patients with COPD, but the maturity status of these cells is unclear. 
      We have quantified the numbers of cells expressing markers associated with DC 
      maturation. METHODS: Lung tissue was obtained at resection for lung cancer from 
      41 patients with COPD (30 current smokers and 11 ex-smokers; 32 steroid-treated 
      patients and 9 steroid-naive patients), 19 ex-smokers without COPD and 9 
      never-smokers without COPD. Tissue sections were immunostained for CD1a to mark 
      immature DCs, and for CD83, fascin, and DC-lysosome-associated membrane protein 
      (DC-LAMP) to delineate mature DCs. RESULTS: The volume density (ie, the volume of 
      DCs as the percentage volume of the airway wall) comprising CD83+ DCs was 
      significantly reduced in patients with COPD (median, 0; range, 0 to 5.1%) vs 
      smokers (median, 2.8%; range, 0 to 10.2%) and never-smokers (median, 1.9%; range, 
      0.8 to 5.1%) without COPD (p = 0.000 and 0.012, respectively). Using a 
      semiquantitative score for the alveolar wall, CD83+ DCs also were decreased in 
      patients with COPD (median, 0; range, 0 to 2%) vs smokers (median, 1%; range, 0 
      to 2%) and never-smokers (median, 1%; range, 0.7 to 2%) without COPD (p = 0.004 
      and 0.04, respectively). No differences were detected in CD83+ DCs between 
      current smokers and ex-smokers with COPD or between steroid-treated and 
      steroid-naive patients. No differences were detected in CD1a+ DCs. Fascin and 
      DC-LAMP were found to have poor specificity for mature DCs. CONCLUSIONS: COPD is 
      associated with decreased numbers of (mature) CD83+ DCs in small airways and 
      alveoli. The relevance of such a reduction on pulmonary immune responses requires 
      further investigation.
FAU - Tsoumakidou, Maria
AU  - Tsoumakidou M
AD  - Departments of Thoracic Medicine, University Hospital of Heraklion, Heraklion, 
      Greece; Department of Critical Care Medicine and Pulmonary Services, Evangelismos 
      Hospital, Athens, Greece. Electronic address: tsoumak@yahoo.gr.
FAU - Koutsopoulos, Anastassios V
AU  - Koutsopoulos AV
AD  - Pathology, University Hospital of Heraklion, Heraklion, Greece.
FAU - Tzanakis, Nikolaos
AU  - Tzanakis N
AD  - Departments of Thoracic Medicine, University Hospital of Heraklion, Heraklion, 
      Greece.
FAU - Dambaki, Konstantina
AU  - Dambaki K
AD  - Pathology, University Hospital of Heraklion, Heraklion, Greece.
FAU - Tzortzaki, Eleni
AU  - Tzortzaki E
AD  - Departments of Thoracic Medicine, University Hospital of Heraklion, Heraklion, 
      Greece.
FAU - Zakynthinos, Spyros
AU  - Zakynthinos S
AD  - Department of Critical Care Medicine and Pulmonary Services, Evangelismos 
      Hospital, Athens, Greece.
FAU - Jeffery, Peter K
AU  - Jeffery PK
AD  - Department of Gene Therapy, Imperial College London, London, UK.
FAU - Siafakas, Nikolaos M
AU  - Siafakas NM
AD  - Departments of Thoracic Medicine, University Hospital of Heraklion, Heraklion, 
      Greece.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090522
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
RN  - 0 (Antigens, CD)
RN  - 0 (Carrier Proteins)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Microfilament Proteins)
RN  - 146808-54-0 (fascin)
SB  - IM
MH  - Aged
MH  - Antigens, CD/metabolism
MH  - Carrier Proteins/metabolism
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Humans
MH  - Immunoglobulins/metabolism
MH  - Lung Neoplasms/pathology/surgery
MH  - Lysosomal Membrane Proteins/metabolism
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Microfilament Proteins/metabolism
MH  - Middle Aged
MH  - Pulmonary Disease, Chronic Obstructive/*immunology/metabolism/pathology
MH  - Smoking/immunology/pathology
MH  - Statistics, Nonparametric
MH  - CD83 Antigen
EDAT- 2009/05/26 09:00
MHDA- 2009/10/09 06:00
CRDT- 2009/05/26 09:00
PHST- 2009/05/26 09:00 [entrez]
PHST- 2009/05/26 09:00 [pubmed]
PHST- 2009/10/09 06:00 [medline]
AID - S0012-3692(09)60540-7 [pii]
AID - 10.1378/chest.08-2824 [doi]
PST - ppublish
SO  - Chest. 2009 Sep;136(3):726-733. doi: 10.1378/chest.08-2824. Epub 2009 May 22.