PMID- 19465899
OWN - NLM
STAT- MEDLINE
DCOM- 20091110
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 22
IP  - 9
DP  - 2009 Sep
TI  - t(14;18)-negative follicular lymphomas are associated with a high frequency of 
      BCL6 rearrangement at the alternative breakpoint region.
PG  - 1251-7
LID - 10.1038/modpathol.2009.81 [doi]
AB  - A frequent chromosomal translocation in mature B-cell non-Hodgkin lymphoma 
      affects band 3q27 and results in the deregulation of the B-cell lymphoma 6 (BCL6) 
      gene. Two breakpoint clusters have been described thus far, the major breakpoint 
      region (MBR) and an alternative breakpoint region (ABR) that is located 245-285 
      kb 5' to BCL6. Translocation at the MBR predominates in diffuse large B-cell 
      lymphoma, whereas translocation at the ABR is reported to be frequently 
      associated with grade 3B follicular lymphoma. However, translocation at the ABR 
      has not been studied in a large series of follicular lymphomas, particularly 
      t(14;18)-negative follicular lymphomas. Therefore, we studied BLC6 rearrangements 
      at the MBR and ABR by using break-apart fluorescence in situ hybridization (FISH) 
      probes in 142 cases of follicular lymphomas, including 63 t(14;18)-negative and 
      79 t(14;18)-positive cases. Conventional cytogenetic (karyotype) analysis was 
      also performed in 58 of the 63 t(14;18)-negative cases. BCL6 rearrangement was 
      found in 26% of t(14;18)-negative and 19% of t(14;18)-positive follicular 
      lymphoma. t(14;18)-negative cases showed a high frequency of rearrangement at the 
      ABR (12%) with an ABR/MBR ratio of 0.86, compared with only 5% with an ABR/MBR 
      ratio of 0.36 in the t(14;18)-positive cases. BCL6 rearrangements were found in 
      all grades of follicular lymphoma but were most frequent in grade 3 
      t(14;18)-negative follicular lymphoma (60%). FISH analysis had a higher 
      sensitivity for detecting BCL6 rearrangements than conventional cytogenetics. In 
      conclusion, BCL6 rearrangements occur at a similar frequency in t(14;18)-negative 
      follicular lymphoma and diffuse large B-cell lymphoma. However, t(14;18)-negative 
      follicular lymphoma appears to have a higher frequency of rearrangement at the 
      ABR compared with t(14;18)-positive follicular lymphoma and diffuse large B-cell 
      lymphoma. Therefore, it is important to perform FISH analysis with ABR to 
      determine possible involvement of BCL6 rearrangement in follicular lymphoma, 
      especially in t(14;18)-negative cases.
FAU - Gu, Keni
AU  - Gu K
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha, NE 68198-3135, USA.
FAU - Fu, Kai
AU  - Fu K
FAU - Jain, Smrati
AU  - Jain S
FAU - Liu, Zhongfen
AU  - Liu Z
FAU - Iqbal, Javeed
AU  - Iqbal J
FAU - Li, Min
AU  - Li M
FAU - Sanger, Warren G
AU  - Sanger WG
FAU - Weisenburger, Dennis D
AU  - Weisenburger DD
FAU - Greiner, Timothy C
AU  - Greiner TC
FAU - Aoun, Patricia
AU  - Aoun P
FAU - Dave, Bhavana J
AU  - Dave BJ
FAU - Chan, Wing C
AU  - Chan WC
LA  - eng
GR  - U01 CA114778/CA/NCI NIH HHS/United States
GR  - U01 CA114778-03/CA/NCI NIH HHS/United States
GR  - 5U01/CA114778/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090522
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (BCL6 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Breakage
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/*genetics
MH  - Lymphoma, Large B-Cell, Diffuse/genetics
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Translocation, Genetic/genetics
PMC - PMC2736319
MID - NIHMS111999
EDAT- 2009/05/26 09:00
MHDA- 2009/11/11 06:00
PMCR- 2010/03/01
CRDT- 2009/05/26 09:00
PHST- 2009/05/26 09:00 [entrez]
PHST- 2009/05/26 09:00 [pubmed]
PHST- 2009/11/11 06:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - S0893-3952(22)02478-4 [pii]
AID - 10.1038/modpathol.2009.81 [doi]
PST - ppublish
SO  - Mod Pathol. 2009 Sep;22(9):1251-7. doi: 10.1038/modpathol.2009.81. Epub 2009 May 
      22.