PMID- 19467264
OWN - NLM
STAT- MEDLINE
DCOM- 20090811
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 160
IP  - 1-2
DP  - 2009 Sep
TI  - Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and 
      RNA viruses in pigs with multiple infections.
PG  - 210-4
LID - 10.1016/j.jviromet.2009.05.010 [doi]
AB  - Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in 
      pigs with multiple infections. These viruses are: porcine circovirus type 2 
      (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and 
      respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine 
      rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible 
      gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be 
      high specificity and sensitivity. In the clinical application, a total of 75 
      field samples were examined by our methods and previously reported methods for 
      PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex 
      PCR and multiplex RT-PCR were higher than those of the previously reported 
      methods. Furthermore, it was confirmed that 24 PCV2 positive samples were 
      co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 
      with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A 
      were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest 
      that the combination of our multiplex PCR and multiplex RT-PCR is useful for 
      rapid and accurate identification of nine major pathogenic viruses in pigs with 
      multiple infections.
FAU - Ogawa, Hirohito
AU  - Ogawa H
AD  - Nippon Institute for Biological Science, Shinmachi, Ome, Tokyo, Japan. 
      hogawa@nibs.or.jp
FAU - Taira, Osamu
AU  - Taira O
FAU - Hirai, Takuya
AU  - Hirai T
FAU - Takeuchi, Hiromi
AU  - Takeuchi H
FAU - Nagao, Aki
AU  - Nagao A
FAU - Ishikawa, Yoshiki
AU  - Ishikawa Y
FAU - Tuchiya, Kotaro
AU  - Tuchiya K
FAU - Nunoya, Tetsuo
AU  - Nunoya T
FAU - Ueda, Susumu
AU  - Ueda S
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090523
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Animals
MH  - DNA Viruses/*isolation & purification
MH  - Polymerase Chain Reaction/*methods
MH  - RNA Viruses/*isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
MH  - Virus Diseases/diagnosis/*veterinary
EDAT- 2009/05/27 09:00
MHDA- 2009/08/12 09:00
CRDT- 2009/05/27 09:00
PHST- 2009/01/22 00:00 [received]
PHST- 2009/05/08 00:00 [revised]
PHST- 2009/05/12 00:00 [accepted]
PHST- 2009/05/27 09:00 [entrez]
PHST- 2009/05/27 09:00 [pubmed]
PHST- 2009/08/12 09:00 [medline]
AID - S0166-0934(09)00244-4 [pii]
AID - 10.1016/j.jviromet.2009.05.010 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Sep;160(1-2):210-4. doi: 10.1016/j.jviromet.2009.05.010. 
      Epub 2009 May 23.