PMID- 19467664
OWN - NLM
STAT- MEDLINE
DCOM- 20100106
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 46
DP  - 2009 Nov 13
TI  - Development of a rapid, multi-class method for the confirmatory analysis of 
      anti-inflammatory drugs in bovine milk using liquid chromatography tandem mass 
      spectrometry.
PG  - 8132-40
LID - 10.1016/j.chroma.2009.04.078 [doi]
AB  - A rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been 
      developed and validated for the simultaneous identification, confirmation and 
      quantitation of seven licensed anti-inflammatory drugs (AIDs) in bovine milk. The 
      method was validated in accordance with the criteria defined in Commission 
      Decision 2002/657/EC. Two classes of AIDs were investigated, corticosteroids and 
      non-steroidal anti-inflammatory drugs (NSAIDs). The developed method is capable 
      of detecting and confirming dexamethasone (DXM), betamethasone (BTM), 
      prednisolone (PRED), tolfenamic acid (TLF), 5-hydroxy flunixin (5-OH-FLU), 
      meloxicam (MLX) and 4-methyl amino antipyrine (4-MAA) at their associated maximum 
      residue limits (MRLs). These compounds represent all the corticosteroids and 
      NSAIDs licensed for use in bovine animals producing milk for human consumption. 
      These compounds have never been analysed before in the same method and also 
      4-methyl amino antipyrine has never been analysed with the other licensed NSAIDs. 
      The method can be considered rapid as permits the analysis of up to 30 samples in 
      one day. Milk samples are extracted with acetonitrile; sodium chloride is added 
      to aid partition of the milk and acetonitrile mixture. The acetonitrile extract 
      is then subjected to liquid-liquid purification by the addition of hexane. The 
      purified extract is finally evaporated to dryness and reconstituted in a 
      water/acetonitrile mixture and determination is carried out by LC-MS/MS. Decision 
      limit (CCalpha) values and detection capability (CCbeta) values have been 
      established for each compound.
FAU - Malone, E M
AU  - Malone EM
AD  - The State Laboratory, Young's Cross, Celbridge, Co Kildare, Ireland. 
      edward.malone@statelab.ie
FAU - Dowling, G
AU  - Dowling G
FAU - Elliott, C T
AU  - Elliott CT
FAU - Kennedy, D G
AU  - Kennedy DG
FAU - Regan, L
AU  - Regan L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090504
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*chemistry
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Milk/*chemistry
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2009/05/27 09:00
MHDA- 2010/01/07 06:00
CRDT- 2009/05/27 09:00
PHST- 2009/01/04 00:00 [received]
PHST- 2009/03/31 00:00 [revised]
PHST- 2009/04/24 00:00 [accepted]
PHST- 2009/05/27 09:00 [entrez]
PHST- 2009/05/27 09:00 [pubmed]
PHST- 2010/01/07 06:00 [medline]
AID - S0021-9673(09)00668-2 [pii]
AID - 10.1016/j.chroma.2009.04.078 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 Nov 13;1216(46):8132-40. doi: 10.1016/j.chroma.2009.04.078. 
      Epub 2009 May 4.