PMID- 19469586
OWN - NLM
STAT- MEDLINE
DCOM- 20091208
LR  - 20161018
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 28
IP  - 9
DP  - 2009 Sep
TI  - Advanced fluorescence in situ hybridization to localize and quantify gene 
      expression in Japanese medaka (Oryzias latipes) exposed to endocrine-disrupting 
      compounds.
PG  - 1951-62
LID - 10.1897/08-574.1 [doi]
AB  - In an earlier study, we described the development of fluorescence in situ 
      hybridization (FISH) using confocal microscopy to localize and quantify gene 
      expression in fish. Here, we report the results of FISH application to 
      investigate effects of model endocrine-disrupting chemicals (EDCs), 
      17alpha-ethinylestradiol (EE2) and 17beta-trenbolone (TB), on expressions of 
      EDC-responsive genes in Japanese medaka (Oryzias latipes) at the cellular/tissue 
      level paired with histological observation. Gene expressions of vitellogenin-II 
      (Vit-II), androgen receptor (AR), and cytochrome P450 gonadal aromatase (CYP19a) 
      were determined after exposure to 5, 50, or 500 ng/L of EE2 or 50, 500, or 5,000 
      ng/L of TB for 7 d. Exposure to the greatest concentration of EE2 or TB 
      significantly reduced fecundity and caused histological alterations in gonads. 
      17alpha-Ethinylestradiol induced Vit-II expression in both male gonads and liver 
      relative to controls and resulted in greater intensity of hematoxylin staining in 
      hepatocytes, which was significantly correlated with Vit-II induction in liver. 
      When exposed to EE2 at less than 50 ng/L, CYP19a expression associated with early 
      stage oocytes was greater than that in controls. However, at 500 ng/L, this trend 
      was reversed. The greater Vit-II expression in testis from all EE2 groups, and 
      the lesser expression of CYP19a in ovaries from the 500 ng/L group, likely is 
      related to changes in the number of cells in which these genes are predominantly 
      expressed rather than to an increase in expression per cell. 17beta-Trenbolone 
      significantly induced AR expression in ovaries but did not alter AR expression in 
      female liver. It was concluded that FISH combined with histology enables advanced 
      elucidation of molecular effects of chemicals by associating changes in gene 
      expression with certain tissues and/or cell types and allows these changes to be 
      related to histological effects.
FAU - Park, June-Woo
AU  - Park JW
AD  - Department of Zoology, National Food Safety and Toxicology Center, Michigan State 
      University, East Lansing, Michigan 48824, USA. jpark41@utk.edu
FAU - Tompsett, Amber R
AU  - Tompsett AR
FAU - Zhang, Xiaowei
AU  - Zhang X
FAU - Newsted, John L
AU  - Newsted JL
FAU - Jones, Paul D
AU  - Jones PD
FAU - Au, Doris W T
AU  - Au DW
FAU - Kong, Richard
AU  - Kong R
FAU - Wu, Rudolf S S
AU  - Wu RS
FAU - Giesy, John P
AU  - Giesy JP
FAU - Hecker, Markus
AU  - Hecker M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Endocrine Disruptors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Vitellogenins)
RN  - 423D2T571U (Ethinyl Estradiol)
RN  - EC 1.14.14.1 (Aromatase)
RN  - RUD5Y4SV0S (Trenbolone Acetate)
SB  - IM
MH  - Animals
MH  - Aromatase/genetics
MH  - Endocrine Disruptors/*toxicity
MH  - Ethinyl Estradiol/toxicity
MH  - Female
MH  - Gene Expression Profiling
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Oryzias/*metabolism
MH  - RNA, Messenger/*analysis
MH  - Receptors, Androgen/genetics
MH  - Trenbolone Acetate/toxicity
MH  - Vitellogenins/genetics
EDAT- 2009/05/28 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/28 09:00
PHST- 2008/11/10 00:00 [received]
PHST- 2009/04/13 00:00 [accepted]
PHST- 2009/05/28 09:00 [entrez]
PHST- 2009/05/28 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 08-574 [pii]
AID - 10.1897/08-574.1 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2009 Sep;28(9):1951-62. doi: 10.1897/08-574.1.