PMID- 19470254
OWN - NLM
STAT- MEDLINE
DCOM- 20090811
LR  - 20090527
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 11
IP  - 5
DP  - 2009 May
TI  - [Relationship of levels of CD4(+)CD25(+) regulatory T cells and expression of 
      Foxp3 mRNA in peripheral blood with serum immunoglobulin E level in children with 
      bronchiolitis].
PG  - 349-53
AB  - OBJECTIVE: To study the roles of CD4(+)CD25(+) regulatory T cells and Foxp3 mRNA 
      in peripheral blood as well as serum total immunoglobulin E (IgE) in the 
      pathogenesis of bronchiolitis caused by respiratory syncytial virus (RSV). 
      METHODS: The proportion of CD4(+)CD25(+) regulatory T cells and expression of 
      Foxp3 mRNA in peripheral blood, and total serum IgE level were tested by flow 
      cytometry, RT-PCR and ELISA respectively in 57 children with RSV bronchiolitis 
      (26 atopic patients and 31 nonatopic patients). Twenty five healthy children were 
      used as the control group. RESULTS: The proportion of CD4(+)CD25(+) regulatory T 
      cells in peripheral blood in children with bronchiolitis, either in the atopic 
      (7.7+/- 1.6%)or the nonatopic group (8.8+/- 2.1%), was significantly lower than 
      that in the control group (10.5+/- 1.6%) (P< 0.01). Foxp3 mRNA expression in 
      peripheral blood was significantly lower in both atopic and nonatopic children 
      with bronchiolitis than that in the control group (P< 0.01). Significantly 
      increased total serum IgE level was noted in both atopic (241.2+/- 102.5 IU/mL) 
      and nonatopic children (125.5+/- 63.2 IU/mL) with bronchiolitis compared with 
      that in the control group (27.2+/- 10.5 IU/ml) (P< 0.01). There were significant 
      differences in the proportion of CD4(+)CD25(+) regulatory T cells and Foxp3 mRNA 
      expression in peripheral blood (P< 0.05) as well as total serum IgE level (P< 
      0.01) between the atopic and the nonatopic group. The proportion of CD4(+)CD25(+) 
      regulatory T cells (r=-0.70, P< 0.01) and Foxp3 mRNA expression in peripheral 
      blood (r=-0.79, P< 0.01) were closely negatively correlated to total serum IgE 
      level. CONCLUSIONS: Both the proportion of CD4(+)CD25(+) regulatory T cells and 
      Foxp3 mRNA expression in peripheral blood were reduced, in contrast, the total 
      serum IgE level increased in children with RSV bronchiolitis. This suggested that 
      CD4(+)CD25(+) regulatory T cells and Foxp3 mRNA together with IgE participated in 
      the pathogenesis of RSV bronchiolitis.
FAU - Zhu, Ya-Fei
AU  - Zhu YF
AD  - Department of Pediatrics, Fifth Affiliated Hospital of Wenzhou Medical 
      College/Lishui Central Hospital, Lishui, Zhejiang, China. lszhuyafei@sina.com
FAU - Zhu, Jian-Yang
AU  - Zhu JY
FAU - Li, Wei-Wu
AU  - Li WW
FAU - Lan, Chen-Fu
AU  - Lan CF
FAU - Fang, Jing
AU  - Fang J
FAU - Lin, Hai-Ling
AU  - Lin HL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Bronchiolitis/etiology/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Female
MH  - Forkhead Transcription Factors/*genetics
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Infant
MH  - Male
MH  - RNA, Messenger/*blood
MH  - Respiratory Syncytial Virus Infections/etiology/*immunology
MH  - T-Lymphocytes, Regulatory/*physiology
EDAT- 2009/05/28 09:00
MHDA- 2009/08/12 09:00
CRDT- 2009/05/28 09:00
PHST- 2009/05/28 09:00 [entrez]
PHST- 2009/05/28 09:00 [pubmed]
PHST- 2009/08/12 09:00 [medline]
AID - 1008-8830(2009)05-0349-05 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2009 May;11(5):349-53.