PMID- 19473328
OWN - NLM
STAT- MEDLINE
DCOM- 20091028
LR  - 20220408
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 59
IP  - 6
DP  - 2009 Sep
TI  - Compensation and interaction between RISBZ1 and RPBF during grain filling in 
      rice.
PG  - 908-20
LID - 10.1111/j.1365-313X.2009.03925.x [doi]
AB  - The rice (Oryza sativa L.) basic leucine Zipper factor RISBZ1 and rice prolamin 
      box binding factor (RPBF) are transcriptional activators of rice seed storage 
      protein (SSP) genes in vivo. To ascertain the functions of these trans-activators 
      in seed development, knock-down (KD) transgenic rice plants were generated in 
      which the accumulation of RISBZ1 and RPBF was reduced in an endosperm-specific 
      manner by co-suppression (KD-RISBZ1 and KD-RPBF). The accumulation of most SSPs 
      changed little between individual KD mutants and wild-type plants, whereas a 
      double KD mutant (KD-RISBZ1/KD-RPBF) resulted in a significant reduction of most 
      SSP gene expression and accumulation. The reduction of both trans-activators also 
      caused a greater reduction in seed starch accumulation than individual KD 
      mutants. Storage lipids were accumulated at reduced levels in KD-RISBZ1 and 
      KD-RISBZ1/KD-RPBF seeds. KD-RPBF and KD-RISBZ1/KD-RPBF seeds exhibited 
      multi-layered aleurone cells. Gene expression of DEFECTIVE KERNEL1 (OsDEK1), 
      CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was 
      decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 
      and RPBF. These phenotypes suggest that combinatorial interactions between RISBZ1 
      and RPBF play an essential role during grain filling. The functional redundancy 
      and compensation between RISBZ1 and RPBF possibly account for weak effects on the 
      SSP levels in single KD mutants, and help maintain various processes during seed 
      development in rice. Physical interaction between RISBZ1 and RPBF may ensure that 
      these processes are carried out properly.
FAU - Kawakatsu, Taiji
AU  - Kawakatsu T
AD  - Transgenic Crop Research and Development Centre, National Institute of 
      Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
FAU - Yamamoto, Masayuki P
AU  - Yamamoto MP
FAU - Touno, Satoru M
AU  - Touno SM
FAU - Yasuda, Hiroshi
AU  - Yasuda H
FAU - Takaiwa, Fumio
AU  - Takaiwa F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090518
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lipids)
RN  - 0 (PBF protein, plant)
RN  - 0 (Plant Proteins)
RN  - 0 (RISBZ1 protein, Oryza sativa)
RN  - 0 (RNA, Plant)
RN  - 0 (Seed Storage Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 9005-25-8 (Starch)
SB  - IM
MH  - Basic-Leucine Zipper Transcription Factors/genetics/*metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Gene Knockdown Techniques
MH  - Lipids/analysis
MH  - Mutagenesis, Insertional
MH  - Oryza/*genetics/metabolism
MH  - Plant Proteins/genetics/*metabolism
MH  - Plants, Genetically Modified/genetics/metabolism
MH  - RNA Interference
MH  - RNA, Plant/genetics
MH  - Seed Storage Proteins/metabolism
MH  - Seeds/genetics/*growth & development
MH  - Starch/analysis
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2009/05/29 09:00
MHDA- 2009/10/29 06:00
CRDT- 2009/05/29 09:00
PHST- 2009/05/29 09:00 [entrez]
PHST- 2009/05/29 09:00 [pubmed]
PHST- 2009/10/29 06:00 [medline]
AID - TPJ3925 [pii]
AID - 10.1111/j.1365-313X.2009.03925.x [doi]
PST - ppublish
SO  - Plant J. 2009 Sep;59(6):908-20. doi: 10.1111/j.1365-313X.2009.03925.x. Epub 2009 
      May 18.