PMID- 19473452
OWN - NLM
STAT- MEDLINE
DCOM- 20091215
LR  - 20161125
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 38
IP  - 9
DP  - 2009 Oct
TI  - WNT-5A, but not matrix metalloproteinase 3 or beta-catenin protein, expression is 
      related to early stages of lip carcinogenesis.
PG  - 708-15
LID - 10.1111/j.1600-0714.2009.00756.x [doi]
AB  - BACKGROUND: Oncogenic Wnt/beta-catenin signaling occurs in numerous types of 
      cancers, but little is known about the role of the Wnt protein family member, 
      WNT-5A, in lip carcinogenesis. The aim of this study was to investigate WNT-5A, 
      beta-catenin, and matrix metalloproteinase (MMP)-3 protein expression in actinic 
      cheilitis (AC), and lip squamous cell carcinoma (LSCC). METHODS: Twenty-one cases 
      of AC, and fifty-one cases of LSCC were analyzed, with normal lip mucosa used as 
      a control. Qualitative and semi-quantitative analyses of WNT-5A, beta-catenin, 
      and MMP-3 immunostaining pattern and cellular distribution were performed. 
      RESULTS: WNT-5A was observed in more than 50% of the cells, scattered in all 
      layers of AC, in contrast to the absence of immunostaining in normal lip mucosa. 
      AC presented a higher level of WNT-5A expression than LSCC (P = 0.0289, Fisher 
      test), while MMP-3 immunoexpression was statistically more significant in LSCC 
      than in AC (P = 0.0285, Fisher test). Immunolabeling of beta-catenin protein was 
      differentially distributed between samples; the majority of AC cases (61.90%) 
      demonstrated a membranous-cytoplasmic pattern, while a considerable number of 
      LSCC cases (29.41%) revealed a cytoplasmic pattern, instead of the usual 
      membranous pattern. CONCLUSIONS: The present results suggest that WNT-5A may be 
      an important marker during initial events of AC malignant transformation, in 
      which non-canonical and canonical Wnt/beta-catenin signaling pathways could be 
      involved. Additionally, WNT-5A might recruit other events in LSCC, such as MMP-3 
      protein synthesis, as its presence is increased in established malignant 
      processes without beta-catenin dependency.
FAU - Xavier, Flavia Calo Aquino
AU  - Xavier FC
AD  - Department of Oral Pathology, School of Dentistry, University of Sao Paulo, Sao 
      Paulo, Brazil. f.calo@usp.br
FAU - Rodini, Camila Oliveira
AU  - Rodini CO
FAU - Ramalho, Luciana Maria Pedreira
AU  - Ramalho LM
FAU - Mantesso, Andrea
AU  - Mantesso A
FAU - Nunes, Fabio Daumas
AU  - Nunes FD
LA  - eng
PT  - Journal Article
DEP - 20090311
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (WNT5A protein, human)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt-5a Protein)
RN  - 0 (beta Catenin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Membrane/metabolism
MH  - Cell Nucleus/metabolism
MH  - Cell Transformation, Neoplastic
MH  - Cheilitis/etiology/*metabolism
MH  - Cytoplasm/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Lip Neoplasms/*metabolism/pathology
MH  - Matrix Metalloproteinase 3/*biosynthesis
MH  - Mouth Mucosa/metabolism/pathology
MH  - Proto-Oncogene Proteins/*biosynthesis/physiology
MH  - Retrospective Studies
MH  - Signal Transduction
MH  - Sunlight/adverse effects
MH  - Wnt Proteins/*biosynthesis/physiology
MH  - Wnt-5a Protein
MH  - beta Catenin/*biosynthesis
EDAT- 2009/05/29 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/29 09:00
PHST- 2009/05/29 09:00 [entrez]
PHST- 2009/05/29 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - JOP756 [pii]
AID - 10.1111/j.1600-0714.2009.00756.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2009 Oct;38(9):708-15. doi: 10.1111/j.1600-0714.2009.00756.x. 
      Epub 2009 Mar 11.