PMID- 19473542
OWN - NLM
STAT- MEDLINE
DCOM- 20090713
LR  - 20211020
IS  - 1471-2334 (Electronic)
IS  - 1471-2334 (Linking)
VI  - 9
DP  - 2009 May 27
TI  - Potential plasma markers of Type 1 and Type 2 leprosy reactions: a preliminary 
      report.
PG  - 75
LID - 10.1186/1471-2334-9-75 [doi]
AB  - BACKGROUND: The clinical management of leprosy Type 1 (T1R) and Type 2 (T2R) 
      reactions pose challenges mainly because they can cause severe nerve injury and 
      disability. No laboratory test or marker is available for the diagnosis or 
      prognosis of leprosy reactions. This study simultaneously screened plasma factors 
      to identify circulating biomarkers associated with leprosy T1R and T2R among 
      patients recruited in Goiania, Central Brazil. METHODS: A nested case-control 
      study evaluated T1R (n = 10) and TR2 (n = 10) compared to leprosy patients 
      without reactions (n = 29), matched by sex and age-group (+/- 5 years) and 
      histopathological classification. Multiplex bead based technique provided 
      profiles of 27 plasma factors including 16 pro inflammatory cytokines: tumor 
      necrosis factor-alpha (TNF-alpha), Interferon-gamma (IFN-gamma), interleukin 
      (IL)- IL12p70, IL2, IL17, IL1 beta, IL6, IL15, IL5, IL8, macrophage inflammatory 
      protein (MIP)-1 alpha (MIP1alpha), 1 beta (MIP1beta), regulated upon activation 
      normal T-cell expressed and secreted (RANTES), monocyte chemoattractrant protein 
      1 (MCP1), CC-chemokine 11 (CCL11/Eotaxin), CXC-chemokine 10 (CXCL10/IP10); 4 anti 
      inflammatory interleukins: IL4, IL10, IL13, IL1Ralpha and 7 growth factors: IL7, 
      IL9, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony 
      stimulating factor (GM-CSF), platelet-derived growth factor BB (PDGF BB), basic 
      fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF). 
      RESULTS: Elevations of plasma CXCL10 (P = 0.004) and IL6 (p = 0.013) were 
      observed in T1R patients compared to controls without reaction. IL6 (p = 0.05), 
      IL7 (p = 0.039), and PDGF-BB (p = 0.041) were elevated in T2R. RANTES and GMCSF 
      were excluded due to values above and below detection limit respectively in all 
      samples. CONCLUSION: Potential biomarkers of T1R identified were CXCL10 and IL6 
      whereas IL7, PDGF-BB and IL6, may be laboratory markers of TR2. Additional 
      studies on these biomarkers may help understand the immunopathologic mechanisms 
      of leprosy reactions and indicate their usefulness for the diagnosis and for the 
      clinical management of these events.
FAU - Stefani, Mariane M
AU  - Stefani MM
AD  - Tropical Pathology and Public Health Institute, Federal University of Goias, GO, 
      Brazil. mariane.stefani@pq.cnpq.br
FAU - Guerra, Jackeline G
AU  - Guerra JG
FAU - Sousa, Ana Lucia M
AU  - Sousa AL
FAU - Costa, Mauricio B
AU  - Costa MB
FAU - Oliveira, Maria Leide W
AU  - Oliveira ML
FAU - Martelli, Celina T
AU  - Martelli CT
FAU - Scollard, David M
AU  - Scollard DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090527
PL  - England
TA  - BMC Infect Dis
JT  - BMC infectious diseases
JID - 100968551
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers/*blood
MH  - Brazil
MH  - Case-Control Studies
MH  - Cytokines/*blood
MH  - Female
MH  - Humans
MH  - Leprosy/*blood
MH  - Male
MH  - Middle Aged
MH  - Young Adult
PMC - PMC2696458
EDAT- 2009/05/29 09:00
MHDA- 2009/07/14 09:00
PMCR- 2009/05/27
CRDT- 2009/05/29 09:00
PHST- 2009/01/07 00:00 [received]
PHST- 2009/05/27 00:00 [accepted]
PHST- 2009/05/29 09:00 [entrez]
PHST- 2009/05/29 09:00 [pubmed]
PHST- 2009/07/14 09:00 [medline]
PHST- 2009/05/27 00:00 [pmc-release]
AID - 1471-2334-9-75 [pii]
AID - 10.1186/1471-2334-9-75 [doi]
PST - epublish
SO  - BMC Infect Dis. 2009 May 27;9:75. doi: 10.1186/1471-2334-9-75.