PMID- 19475651
OWN - NLM
STAT- MEDLINE
DCOM- 20100614
LR  - 20131121
IS  - 1098-1063 (Electronic)
IS  - 1050-9631 (Linking)
VI  - 20
IP  - 4
DP  - 2010 Apr
TI  - Modulatory metaplasticity induced by pregnenolone sulfate in the rat hippocampus: 
      a leftward shift in LTP/LTD-frequency curve.
PG  - 499-512
LID - 10.1002/hipo.20649 [doi]
AB  - We recently have found that an acute application of the neurosteroid pregnenolone 
      sulfate (PREGS) at 50 muM to rat hippocampal slices induces a long-lasting 
      potentiation (LLP(PREGS)) via a sustained ERK2/CREB activation at 
      perforant-path/granule-cell synapses in the dentate gyrus. This study is a follow 
      up to investigate whether the expression of LLP(PREGS) influences subsequent 
      frequency-dependent synaptic plasticity. Conditioning electric stimuli (CS) at 
      0.1-200 Hz were given to the perforant-path of rat hippocampal slices expressing 
      LLP(PREGS) to induce long-term potentiation (LTP) and long-term depression (LTD). 
      The largest LTP was induced at about 20 Hz-CS, which is normally a subthreshold 
      frequency, and the largest LTD at 0.5 Hz-CS, resulting in a leftward-shift of the 
      LTP/LTD-frequency curve. Furthermore, the level of LTP at 100 Hz-CS was 
      significantly attenuated to give band-pass filter characteristics of LTP 
      induction with a center frequency of about 20 Hz. The LTP induced by 20 Hz-CS 
      (termed 20 Hz-LTP) was found to be postsynaptic origin and dependent on L-type 
      voltage-gated calcium channel (L-VGCC) but not on N-methyl-D-aspartate receptor 
      (NMDAr). Moreover, the induction of 20 Hz-LTP required a sustained activation of 
      ERK2 that had been triggered by PREGS. In conclusion, the transient elevation of 
      PREGS is suggested to induce a modulatory metaplasticity through a sustained 
      activation of ERK2 in an L-VGCC dependent manner.
CI  - (c) 2009 Wiley-Liss, Inc.
FAU - Chen, Ling
AU  - Chen L
AD  - Laboratory of Reproductive Medicine, Department of Physiology, Nanjing Medical 
      University, Nanjing, China. lingchen@njmu.edu.cn
FAU - Cai, Weiyan
AU  - Cai W
FAU - Chen, Lei
AU  - Chen L
FAU - Zhou, Rong
AU  - Zhou R
FAU - Furuya, Kishio
AU  - Furuya K
FAU - Sokabe, Masahiro
AU  - Sokabe M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hippocampus
JT  - Hippocampus
JID - 9108167
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 73R90F7MQ8 (Pregnenolone)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Calcium Channels, L-Type/metabolism
MH  - Electric Stimulation
MH  - Hippocampus/*drug effects/physiology
MH  - Long-Term Potentiation/*drug effects/physiology
MH  - Long-Term Synaptic Depression/*drug effects/physiology
MH  - Male
MH  - Neurons/drug effects/physiology
MH  - Pregnenolone/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Synapses/drug effects/physiology
EDAT- 2009/05/29 09:00
MHDA- 2010/06/15 06:00
CRDT- 2009/05/29 09:00
PHST- 2009/05/29 09:00 [entrez]
PHST- 2009/05/29 09:00 [pubmed]
PHST- 2010/06/15 06:00 [medline]
AID - 10.1002/hipo.20649 [doi]
PST - ppublish
SO  - Hippocampus. 2010 Apr;20(4):499-512. doi: 10.1002/hipo.20649.