PMID- 19477020
OWN - NLM
STAT- MEDLINE
DCOM- 20091203
LR  - 20141120
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 131
IP  - 3-4
DP  - 2009 Oct 15
TI  - Ex vivo generation of mature equine monocyte-derived dendritic cells.
PG  - 259-67
LID - 10.1016/j.vetimm.2009.04.019 [doi]
AB  - Dendritic cells (DCs) are innate immune cells specialized in antigen detection 
      and presentation. They perform an essential role in initiating and guiding the 
      immune response, the direction of which largely depends upon the activation state 
      of the DCs. The objective of this study was to generate mature equine 
      monocyte-derived DCs and, in doing so, to develop a method for measuring the 
      activation state of these cells. Equine DCs were stimulated with UV-inactivated 
      Escherichia coli (E. coli), and the activation status was measured by analyzing 
      cell surface marker expression, cytokine production, and endocytic capacity. 
      Comparisons for each parameter measured were performed between macrophages, 
      non-stimulated DCs and stimulated DCs. Equine monocyte-derived DCs may be 
      distinguished from macrophages based on cell surface expression of MHC class II 
      (p<0.0001) and CD206 (p<0.0001), their capacity for endocytosis of FITC-dextran 
      (p<0.05), and production of TNF-alpha upon stimulation (p<0.001). Furthermore, 
      stimulated DCs can be distinguished from non-stimulated DCs based on increased 
      cell surface expression of MHC class II (p<0.0001) and upregulation of 
      pro-inflammatory cytokine mRNA, particularly IL-12/IL-23p40 (p<0.05) and IL-23p19 
      (p<0.05). The ability to measure DC activation state will facilitate future 
      investigations of equine DC function.
FAU - Cavatorta, Derek J
AU  - Cavatorta DJ
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Cornell 
      University, Ithaca, NY, USA.
FAU - Erb, Hollis N
AU  - Erb HN
FAU - Flaminio, M Julia B F
AU  - Flaminio MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090504
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, Surface)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Surface/genetics/metabolism
MH  - Base Sequence
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/genetics/metabolism
MH  - DNA Primers/genetics
MH  - Dendritic Cells/cytology/*immunology
MH  - Endocytosis
MH  - Escherichia coli/immunology
MH  - Gene Expression
MH  - Horses/*immunology
MH  - Immunity, Innate
MH  - In Vitro Techniques
MH  - Macrophages/cytology/immunology
MH  - Monocytes/cytology/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Chemokine/genetics/metabolism
EDAT- 2009/05/30 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/30 09:00
PHST- 2009/01/07 00:00 [received]
PHST- 2009/03/26 00:00 [revised]
PHST- 2009/04/22 00:00 [accepted]
PHST- 2009/05/30 09:00 [entrez]
PHST- 2009/05/30 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0165-2427(09)00159-7 [pii]
AID - 10.1016/j.vetimm.2009.04.019 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2009 Oct 15;131(3-4):259-67. doi: 
      10.1016/j.vetimm.2009.04.019. Epub 2009 May 4.