PMID- 19477088
OWN - NLM
STAT- MEDLINE
DCOM- 20091201
LR  - 20191210
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 138
IP  - 3-4
DP  - 2009 Sep 18
TI  - Function of PRRSV GP5 envelope protein by using pseudotyped virus.
PG  - 297-303
LID - 10.1016/j.vetmic.2009.04.013 [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped 
      RNA virus. Virions of PRRSV contain six membrane proteins: the major proteins GP5 
      and M and the minor proteins GP2, GP3, GP4, and E. The GP5 is the major envelope 
      proteins, which was involved in the formation and infectivity of PRRSV by 
      coaction with other membrane proteins. Here, to determine the function of alone 
      GP5 envelope protein in viral entry, we investigated the formation and 
      infectivity of GP5-pseudotyped virus particles. By co-transfection of GP5 
      expression plasmids with murine leukemia virus (MuLV) based retroviral vectors 
      (pHIT60, encoding MuLV Gag-Pol; pHIT111, encoding an MuLV genome with a 
      beta-galactosidase reporter gene) into 293 T cells and analysis of the culture 
      medium using ultracentrifugation, Western blot, and infection assay. We observed 
      that the GP5 envelope protein was incorporated into the MuLV retroviral vectors 
      to generate an pseudotyped murine leukemia virus, which was infectious to PAM and 
      Mack-145 target cells and displayed the same host range with wild-type PRRSV. The 
      infection of the pseudotyped virus on PAM target cells is effectively neutralized 
      by polyclonal antibodies specific for PRRSV or GP5. The results suggested that 
      the GP5 protein may play a key role in the viral entry by interacting with the 
      host cell receptor. The GP5-pseudotyped virus will be useful in the 
      identification of the cellular receptor binding with GP5 protein.
FAU - Xia, Ping an
AU  - Xia Pa
AD  - Henan Agricultural University, Zhengzhou, China. xpa88@163.com
FAU - Dang, Zhan guo
AU  - Dang Zg
FAU - Qiu, Huang
AU  - Qiu H
FAU - Fan, Xu
AU  - Fan X
FAU - Zhou, Bin
AU  - Zhou B
FAU - Cui, Bao an
AU  - Cui Ba
FAU - Chen, Pu yan
AU  - Chen Py
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090419
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Gene Expression Regulation, Viral/physiology
MH  - Humans
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*physiology
MH  - Viral Envelope Proteins/*physiology
EDAT- 2009/05/30 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/30 09:00
PHST- 2008/02/26 00:00 [received]
PHST- 2009/03/18 00:00 [revised]
PHST- 2009/04/14 00:00 [accepted]
PHST- 2009/05/30 09:00 [entrez]
PHST- 2009/05/30 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0378-1135(09)00203-X [pii]
AID - 10.1016/j.vetmic.2009.04.013 [doi]
PST - ppublish
SO  - Vet Microbiol. 2009 Sep 18;138(3-4):297-303. doi: 10.1016/j.vetmic.2009.04.013. 
      Epub 2009 Apr 19.