PMID- 19477173
OWN - NLM
STAT- MEDLINE
DCOM- 20090928
LR  - 20181201
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 315
IP  - 15
DP  - 2009 Sep 10
TI  - Modulation of DNA glycosylase activities in mesenchymal stem cells.
PG  - 2558-67
LID - 10.1016/j.yexcr.2009.05.017 [doi]
AB  - Adipose-tissue derived mesenchymal stem cells (AT-MSCs) are a promising tool for 
      use in cell-based therapies. However, in vitro expansion is required to obtain 
      clinically relevant cell numbers, and this might increase the chance of genomic 
      instability. DNA repair is crucial for maintaining DNA integrity. Here we have 
      compared the initial step of base excision repair in uncultured and cultured 
      AT-MSCs by analysis of base removal activities and expression levels of relevant 
      DNA glycosylases. Uracil, 5-hydroxyuracil and ethenoadenine removal activities 
      were upregulated in cultured cells compared to uncultured cells. In contrast, 
      both the 8-oxo-7,8-dihydroguanine (8-oxoG) removal activity and the concentration 
      of 8-oxoG bases in the DNA were reduced in the cultured cells. Gene expression 
      analysis showed no substantial changes in mRNA expression. The glycosylase 
      activities remained stable through at least 12 passages, suggesting that DNA 
      repair is proficient through the period required for in vitro expansion of 
      AT-MSCs to clinically relevant numbers.
FAU - Hildrestrand, Gunn A
AU  - Hildrestrand GA
AD  - Centre for Molecular Biology and Neuroscience and Institute of Medical 
      Microbiology, Rikshospitalet University Hospital, Oslo, Norway.
FAU - Duggal, Shivali
AU  - Duggal S
FAU - Bjoras, Magnar
AU  - Bjoras M
FAU - Luna, Luisa
AU  - Luna L
FAU - Brinchmann, Jan E
AU  - Brinchmann JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090525
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 496-76-4 (5-hydroxyuracil)
RN  - 5614-64-2 (8-hydroxyguanine)
RN  - 56HH86ZVCT (Uracil)
RN  - 5Z93L87A1R (Guanine)
RN  - EC 3.2.2.- (DNA Glycosylases)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Cells, Cultured
MH  - DNA Damage
MH  - DNA Glycosylases/genetics/*metabolism
MH  - *DNA Repair
MH  - Gene Expression Profiling
MH  - Guanine/analogs & derivatives/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Uracil/analogs & derivatives/metabolism
EDAT- 2009/05/30 09:00
MHDA- 2009/09/29 06:00
CRDT- 2009/05/30 09:00
PHST- 2009/02/02 00:00 [received]
PHST- 2009/04/28 00:00 [revised]
PHST- 2009/05/19 00:00 [accepted]
PHST- 2009/05/30 09:00 [entrez]
PHST- 2009/05/30 09:00 [pubmed]
PHST- 2009/09/29 06:00 [medline]
AID - S0014-4827(09)00226-2 [pii]
AID - 10.1016/j.yexcr.2009.05.017 [doi]
PST - ppublish
SO  - Exp Cell Res. 2009 Sep 10;315(15):2558-67. doi: 10.1016/j.yexcr.2009.05.017. Epub 
      2009 May 25.