PMID- 19478527
OWN - NLM
STAT- MEDLINE
DCOM- 20090811
LR  - 20090626
IS  - 1423-0100 (Electronic)
IS  - 0300-5526 (Linking)
VI  - 52
IP  - 3
DP  - 2009
TI  - TAT peptides mediated small interfering RNA delivery to Huh-7 cells and 
      efficiently inhibited hepatitis C virus RNA replication.
PG  - 135-40
LID - 10.1159/000220597 [doi]
AB  - OBJECTIVE: To observe whether or not the small-interfering RNA (siRNA) that 
      conjugated with human immunodeficiency virus type 1 (HIV-1) TAT(47-57) peptides 
      can enter Huh-7 cells and efficiently silence hepatitis C virus (HCV) infection 
      in cell culture. METHODS: siRNA targeting the highly conserved stem loop IV of 
      the HCV 5' untranslated region (5'UTR) was conjugated to TAT(47-57) peptides via 
      the crosslinker sulfosuccinimidyl-4-(p-maleimidophenyl)-butyrate, and then the 
      conjugates were added to the Huh-7 cell culture. Firefly luciferase activity and 
      HCV RNA were assessed using a luciferase assay reagent and real-time reverse 
      transcript polymerase chain reaction, respectively. RESULTS: The expression of 
      firefly luciferase in HCV replicons and the concentration of HCV RNA were 
      downregulated by siRNA-TAT(47-57), and siRNA-TAT(47-57) mediated RNA interfering 
      activity which was directly correlated with increasing concentrations of the 
      siRNA-TAT(47-57) conjugate used. CONCLUSION: Cell-penetrating peptides such as 
      HIV-1 TAT are an effective method for the delivery of siRNA targeted at 5'UTR of 
      HCV in mammalian cells.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Meng, Shuang
AU  - Meng S
AD  - National Center for Clinical Laboratories, Beijing Hospital, Beijing, PR China.
FAU - Wei, Baojun
AU  - Wei B
FAU - Xu, Ruihuan
AU  - Xu R
FAU - Zhang, Kuo
AU  - Zhang K
FAU - Wang, Lunan
AU  - Wang L
FAU - Zhang, Rui
AU  - Zhang R
FAU - Li, Jinming
AU  - Li J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090527
PL  - Switzerland
TA  - Intervirology
JT  - Intervirology
JID - 0364265
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 1.13.12.7 (Luciferases, Firefly)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - *Gene Silencing
MH  - Genes, Reporter
MH  - Hepacivirus/*drug effects
MH  - Humans
MH  - Luciferases, Firefly/genetics/metabolism
MH  - RNA, Small Interfering/genetics/*pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Virus Replication/*drug effects
MH  - tat Gene Products, Human Immunodeficiency Virus/*pharmacology
EDAT- 2009/05/30 09:00
MHDA- 2009/08/12 09:00
CRDT- 2009/05/30 09:00
PHST- 2008/09/25 00:00 [received]
PHST- 2009/03/29 00:00 [accepted]
PHST- 2009/05/30 09:00 [entrez]
PHST- 2009/05/30 09:00 [pubmed]
PHST- 2009/08/12 09:00 [medline]
AID - 000220597 [pii]
AID - 10.1159/000220597 [doi]
PST - ppublish
SO  - Intervirology. 2009;52(3):135-40. doi: 10.1159/000220597. Epub 2009 May 27.