PMID- 1947940
OWN - NLM
STAT- MEDLINE
DCOM- 19911125
LR  - 20131121
IS  - 0891-7035 (Print)
IS  - 0891-7035 (Linking)
VI  - 5
IP  - 2
DP  - 1991 Jun
TI  - The response of the tracheal epithelium to concomitant 
      cis-diamminedichloroplatinum (II) and radiation. An electron microscopic study in 
      rabbits.
PG  - 573-82; discussion 582-3
AB  - The ciliated epithelium of the rabbit trachea was irradiated with daily fractions 
      of 2 Gy up to an accumulated dose of 20 Gy (total dose: 2, 6, 10, 16, or 20 Gy). 
      Fifteen to forty-five minutes before the start of each irradiation 0.3 mg 
      Cis-diamminedichloroplatinum (cis-DDP) was given by intraperitoneal injection to 
      each rabbit. Examinations were carried out 1-10 days after each fractionation 
      schedule, when specimens were taken for morphological investigations. Scanning 
      electron microscope (SEM) examination showed a gradual development of ciliary 
      damage, from blebs on the cilia to swollen tips, broken and bent cilia and 
      finally an epithelial injury with areas free from cilia, and a surface covered 
      with microvilli-like structures. SEM also showed cell loss, and remnants of dead 
      cells on the surface together with detritus. By transmission electron microscopy 
      (TEM), ciliary damage, cell death and cell loss of the ciliated cell layer, as 
      well as exfoliation of portions of goblet-like cells on the surface, could be 
      confirmed. Scoring of SEM and TEM micrographs showed that for the tracheal part 
      treated with cis-DDP and radiation, the maximal damage was expressed in the dose 
      group 10 Gy, and above this no further increase in the average reaction occurred. 
      For the part of the trachea only exposed to cis-DDP, the damage increased with 
      the dose. The difference observed speaks for an accelerated proliferation exerted 
      by the radiation.
FAU - Hakansson, C H
AU  - Hakansson CH
AD  - Department of Oncology, University Hospital, Lund, Sweden.
FAU - Albertsson, M
AU  - Albertsson M
FAU - Palmegren, M
AU  - Palmegren M
FAU - Ranstam, J
AU  - Ranstam J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Scanning Microsc
JT  - Scanning microscopy
JID - 8704616
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Animals
MH  - Cilia/drug effects/radiation effects/ultrastructure
MH  - Cisplatin/*pharmacology
MH  - Epithelium/drug effects/radiation effects/ultrastructure
MH  - Injections, Intraperitoneal
MH  - Microscopy, Electron, Scanning
MH  - Rabbits
MH  - Trachea/drug effects/*radiation effects/ultrastructure
MH  - X-Rays
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PST - ppublish
SO  - Scanning Microsc. 1991 Jun;5(2):573-82; discussion 582-3.