PMID- 19479736
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20090706
IS  - 1520-6327 (Electronic)
IS  - 0739-4462 (Linking)
VI  - 71
IP  - 3
DP  - 2009 Jul
TI  - The effect of calorie restriction on growth and development in silkworm, Bombyx 
      mori.
PG  - 159-72
LID - 10.1002/arch.20313 [doi]
AB  - Caloric restriction (CR) is known to extend the life span in different species 
      from yeast to mammals. In this report, a simple organism silkworm (Bombyx mori) 
      was used to study the effect of moderate CR on the growth and development 
      processes of insects. Here we show that an extension of life span upon moderate 
      CR was observed in the silkworm. The total protein level in the 5th instar larvae 
      hemolymph appeared to decline significantly under CR. SDS-PAGE analysis showed 
      that the influence of CR was sex-dependent. The CR effects on female animals were 
      much more significant than on the males. The MALDI-TOF MS study identified 16 
      proteins that expressed differentially among six groups of the male or female 
      larvae fed at different time frequencies. Four of them, storage protein 1 (SP1), 
      arylphorin (SP2), imaginal disk growth factor (IDGF), and 30-kDa lipoprotein, 
      showed significant differences. It was demonstrated that these four proteins were 
      up-regulated when the larvae were over-fed and down-regulated when the larvae 
      were less-fed.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Li, Yijia
AU  - Li Y
AD  - Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. China.
FAU - Chen, Keping
AU  - Chen K
FAU - Yao, Qin
AU  - Yao Q
FAU - Li, Jun
AU  - Li J
FAU - Wang, Yong
AU  - Wang Y
FAU - Liu, Haijun
AU  - Liu H
FAU - Zhang, Chiyu
AU  - Zhang C
FAU - Huang, Guoping
AU  - Huang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Insect Biochem Physiol
JT  - Archives of insect biochemistry and physiology
JID - 8501752
RN  - 0 (Insect Proteins)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Bombyx/*growth & development
MH  - *Caloric Restriction
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Hemolymph/metabolism
MH  - Insect Proteins/metabolism
MH  - Larva/growth & development
MH  - Male
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2009/05/30 09:00
MHDA- 2009/10/02 06:00
CRDT- 2009/05/30 09:00
PHST- 2009/05/30 09:00 [entrez]
PHST- 2009/05/30 09:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - 10.1002/arch.20313 [doi]
PST - ppublish
SO  - Arch Insect Biochem Physiol. 2009 Jul;71(3):159-72. doi: 10.1002/arch.20313.