PMID- 19480690
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 2
DP  - 2009 May 29
TI  - Automated detection of residual cells after sex-mismatched stem-cell 
      transplantation - evidence for presence of disease-marker negative residual 
      cells.
PG  - 12
LID - 10.1186/1755-8166-2-12 [doi]
AB  - BACKGROUND: A new chimerism analysis based on automated interphase fluorescence 
      in situ hybridization (FISH) evaluation was established to detect residual cells 
      after allogene sex-mismatched bone marrow or blood stem-cell 
      transplantation.Cells of 58 patients were characterized as disease-associated due 
      to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous 
      hybridization of gonosome-specific centromeric probes. The automatic slide 
      scanning platform Metafer with its module MetaCyte was used to analyse 3,000 
      cells per sample. RESULTS: Overall 454 assays of 58 patients were analyzed. 13 of 
      58 patients showed residual recipient cells at one stage of more than 4% and 12 
      of 58 showed residual recipient cells less than 4%, respectively. As to be 
      expected, patients of the latter group were associated with a higher survival 
      rate (48 vs. 34 month). In only two of seven patients with disease-marker 
      positive residual cells between 0.1-1.3% a relapse was observed. Besides, 
      disease-marker negative residual cells were found in two patients without relapse 
      at a rate of 2.8% and 3.3%, respectively. CONCLUSION: The definite origin and 
      meaning of disease-marker negative residual cells is still unclear. Overall, with 
      the presented automatic chimerism analysis of interphase FISH slides, a sensitive 
      method for detection of disease-marker positive residual cells is on hand.
FAU - Erlecke, Jorn
AU  - Erlecke J
AD  - Jena University Hospital, Institute of Human Genetics and Anthropology, 
      Kollegiengasse 10, D-07743 Jena, Germany. i8lith@mti.uni-jena.de.
FAU - Hartmann, Isabell
AU  - Hartmann I
FAU - Hoffmann, Martin
AU  - Hoffmann M
FAU - Kroll, Torsten
AU  - Kroll T
FAU - Starke, Heike
AU  - Starke H
FAU - Heller, Anita
AU  - Heller A
FAU - Gloria, Alexander
AU  - Gloria A
FAU - Sayer, Herbert G
AU  - Sayer HG
FAU - Johannes, Tilman
AU  - Johannes T
FAU - Claussen, Uwe
AU  - Claussen U
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Loncarevic, Ivan F
AU  - Loncarevic IF
LA  - eng
PT  - Journal Article
DEP - 20090529
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC2696465
EDAT- 2009/06/02 09:00
MHDA- 2009/06/02 09:01
PMCR- 2009/05/29
CRDT- 2009/06/02 09:00
PHST- 2009/04/27 00:00 [received]
PHST- 2009/05/29 00:00 [accepted]
PHST- 2009/06/02 09:00 [entrez]
PHST- 2009/06/02 09:00 [pubmed]
PHST- 2009/06/02 09:01 [medline]
PHST- 2009/05/29 00:00 [pmc-release]
AID - 1755-8166-2-12 [pii]
AID - 10.1186/1755-8166-2-12 [doi]
PST - epublish
SO  - Mol Cytogenet. 2009 May 29;2:12. doi: 10.1186/1755-8166-2-12.