PMID- 19481219
OWN - NLM
STAT- MEDLINE
DCOM- 20090806
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 27
DP  - 2009 Jul 3
TI  - On-line high-performance liquid chromatography-ultraviolet-nuclear magnetic 
      resonance method of the markers of nerve agents for verification of the Chemical 
      Weapons Convention.
PG  - 5228-34
LID - 10.1016/j.chroma.2009.05.005 [doi]
AB  - This paper details an on-flow liquid chromatography-ultraviolet-nuclear magnetic 
      resonance (LC-UV-NMR) method for the retrospective detection and identification 
      of alkyl alkylphosphonic acids (AAPAs) and alkylphosphonic acids (APAs), the 
      markers of the toxic nerve agents for verification of the Chemical Weapons 
      Convention (CWC). Initially, the LC-UV-NMR parameters were optimized for benzyl 
      derivatives of the APAs and AAPAs. The optimized parameters include stationary 
      phase C(18), mobile phase methanol:water 78:22 (v/v), UV detection at 268nm and 
      (1)H NMR acquisition conditions. The protocol described herein allowed the 
      detection of analytes through acquisition of high quality NMR spectra from the 
      aqueous solution of the APAs and AAPAs with high concentrations of interfering 
      background chemicals which have been removed by preceding sample preparation. The 
      reported standard deviation for the quantification is related to the UV detector 
      which showed relative standard deviations (RSDs) for quantification within 
      +/-1.1%, while lower limit of detection upto 16mug (in mug absolute) for the NMR 
      detector. Finally the developed LC-UV-NMR method was applied to identify the APAs 
      and AAPAs in real water samples, consequent to solid phase extraction and 
      derivatization. The method is fast (total experiment time approximately 2h), 
      sensitive, rugged and efficient.
FAU - Mazumder, Avik
AU  - Mazumder A
AD  - Vertox Laboratory, Defence Research and Development Establishment, Gwalior 
      474002, India.
FAU - Gupta, Hemendra K
AU  - Gupta HK
FAU - Garg, Prabhat
AU  - Garg P
FAU - Jain, Rajeev
AU  - Jain R
FAU - Dubey, Devendra K
AU  - Dubey DK
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090513
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Chemical Warfare Agents)
SB  - IM
MH  - Chemical Warfare Agents/*chemistry
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Online Systems/instrumentation
MH  - Sensitivity and Specificity
EDAT- 2009/06/02 09:00
MHDA- 2009/08/07 09:00
CRDT- 2009/06/02 09:00
PHST- 2009/02/23 00:00 [received]
PHST- 2009/04/16 00:00 [revised]
PHST- 2009/05/07 00:00 [accepted]
PHST- 2009/06/02 09:00 [entrez]
PHST- 2009/06/02 09:00 [pubmed]
PHST- 2009/08/07 09:00 [medline]
AID - S0021-9673(09)00708-0 [pii]
AID - 10.1016/j.chroma.2009.05.005 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 Jul 3;1216(27):5228-34. doi: 10.1016/j.chroma.2009.05.005. 
      Epub 2009 May 13.