PMID- 19481618
OWN - NLM
STAT- MEDLINE
DCOM- 20100105
LR  - 20191210
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 5
IP  - 9
DP  - 2009 Nov
TI  - Cell viability in a wet silica gel.
PG  - 3478-87
LID - 10.1016/j.actbio.2009.05.033 [doi]
AB  - A modified two-step sol-gel route using silicon ethoxide (TEOS) has been used to 
      synthesize amorphous sol-gel-derived silica, which has been successfully used as 
      a cell encapsulation matrix for 3T3 mouse fibroblasts and CRL-2595 epithelial 
      cells due to its non-toxicity. The sol-gel procedure comprised a first, low pH 
      hydrolysis step, followed by a neutral condensation-gelation step. A high 
      water-to-TEOS ratio and the addition of d-glucose as a porogen and source of 
      nutrients were chosen to minimize silica dissolution and improve the 
      biocompatibility of the process. Indeed, the cell integrity in the encapsulation 
      process was preserved by alcohol removal from the starting solution. Cells were 
      then added in a buffered medium, causing rapid gelation and entrapment of the 
      cells within a randomly structured siloxane matrix in the shape of a monolith, 
      which was maintained in the wet state. MTT and alamarBlue assays were used to 
      check the cytotoxicity of the silica gels and the viability of entrapped cells at 
      initial times in contact with silica. To improve cell attachment, cell clumping 
      experiments - where groups of cells were formed - were designed, rendering 
      improved viability. The obtained materials are therefore excellent candidates for 
      designing tissue-culture scaffolds and implantable bioreactors for biomedical 
      applications.
FAU - Nieto, Alejandra
AU  - Nieto A
AD  - Departamento de Quimica Inorganica y Bioinorganica, Facultad de Farmacia, 
      Universidad Complutense de Madrid, E-28040 Madrid, Spain.
FAU - Areva, Sami
AU  - Areva S
FAU - Wilson, Timothy
AU  - Wilson T
FAU - Viitala, Reeta
AU  - Viitala R
FAU - Vallet-Regi, Maria
AU  - Vallet-Regi M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090528
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Biocompatible Materials)
RN  - 0 (Gels)
RN  - 0 (Oxides)
RN  - 0 (Silicon Compounds)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Cell Shape
MH  - *Cell Survival
MH  - Drug Compounding/*methods
MH  - Gels/*chemistry
MH  - Materials Testing
MH  - Mice
MH  - Oxides/*chemistry
MH  - Silicon Compounds/*chemistry
MH  - Silicon Dioxide/chemical synthesis/*chemistry
EDAT- 2009/06/02 09:00
MHDA- 2010/01/06 06:00
CRDT- 2009/06/02 09:00
PHST- 2009/01/24 00:00 [received]
PHST- 2009/04/24 00:00 [revised]
PHST- 2009/05/22 00:00 [accepted]
PHST- 2009/06/02 09:00 [entrez]
PHST- 2009/06/02 09:00 [pubmed]
PHST- 2010/01/06 06:00 [medline]
AID - S1742-7061(09)00247-5 [pii]
AID - 10.1016/j.actbio.2009.05.033 [doi]
PST - ppublish
SO  - Acta Biomater. 2009 Nov;5(9):3478-87. doi: 10.1016/j.actbio.2009.05.033. Epub 
      2009 May 28.