PMID- 19481767
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20181201
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 161
IP  - 2
DP  - 2010 Jun 15
TI  - Adiponectin deficiency promotes the production of inflammatory mediators while 
      severely exacerbating hepatic injury in mice with polymicrobial sepsis.
PG  - 301-11
LID - 10.1016/j.jss.2008.12.021 [doi]
AB  - BACKGROUND: Adiponectin (APN), which is an adipose tissue-derived hormone, is 
      known as an anti-inflammatory cytokine. The effects of APN on the production of 
      inflammatory mediators and hepatic injury during polymicrobial sepsis were 
      evaluated using APN-knockout (KO) mice that had undergone a cecal ligation and 
      puncture (CLP) and rosiglitazone, a selective peroxisome proliferator-activated 
      receptor gamma (PPARgamma) agonist, which increases the plasma APN concentration. 
      MATERIALS AND METHODS: Wild type (WT) and APN-KO mice were underwent CLP. The 
      plasma and hepatic levels of inflammatory mediators, including tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-6 (IL-6), and monocyte chemoattractant 
      protein-1 (MCP-1), were measured before, at 24, and 48 h after CLP. A 
      histological analysis of the liver and the plasma alanine aminotransferase (ALT) 
      levels were examined to evaluate hepatic injury. The plasma levels of 
      inflammatory mediators after CLP with pretreatment of rosiglitazone were compared 
      with those without rosiglitazone. RESULTS: APN deficiency resulted in significant 
      increases in the plasma levels of TNF-alpha, IL-6, and MCP-1 at 24 h after CLP. 
      Hepatic MCP-1 and plasma AST levels in APN-KO mice were significantly higher than 
      those in WT mice at 48 h after CLP. A steatosis change and MCP-1 expressions in 
      hepatocytes were induced in APN-KO mice during sepsis. The administration of 
      rosiglitazone significantly lowered the plasma levels of inflammatory mediators, 
      including TNF-alpha, IL-6, and MCP-1, in WT mice but not in APN-KO mice during 
      sepsis. CONCLUSION: These results suggest that an APN deficiency induces an 
      excessive systemic inflammatory status and exacerbates hepatic injury during 
      polymicrobial sepsis.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Uji, Yoshitaka
AU  - Uji Y
AD  - Department of Surgery, Shiga University of Medical Science, Shiga, Japan.
FAU - Yamamoto, Hiroshi
AU  - Yamamoto H
FAU - Maeda, Kazuhisa
AU  - Maeda K
FAU - Tsuchihashi, Hiroshi
AU  - Tsuchihashi H
FAU - Akabori, Hiroya
AU  - Akabori H
FAU - Shimizu, Tomoharu
AU  - Shimizu T
FAU - Endo, Yoshihiro
AU  - Endo Y
FAU - Shimomura, Iichiro
AU  - Shimomura I
FAU - Tani, Tohru
AU  - Tani T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090114
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Adiponectin)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 05V02F2KDG (Rosiglitazone)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Adiponectin/*deficiency
MH  - Alanine Transaminase/blood/metabolism
MH  - Animals
MH  - Disease Models, Animal
MH  - Fibrinolytic Agents/therapeutic use
MH  - Inflammation/blood/physiopathology
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-6/blood
MH  - Liver/enzymology/injuries/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Punctures
MH  - Receptors, CCR2/blood
MH  - Rosiglitazone
MH  - Sepsis/*etiology/microbiology/physiopathology
MH  - Shock, Septic/*etiology/microbiology
MH  - Thiazolidinediones/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2009/06/02 09:00
MHDA- 2010/06/04 06:00
CRDT- 2009/06/02 09:00
PHST- 2008/10/21 00:00 [received]
PHST- 2008/12/12 00:00 [revised]
PHST- 2008/12/15 00:00 [accepted]
PHST- 2009/06/02 09:00 [entrez]
PHST- 2009/06/02 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
AID - S0022-4804(08)01580-1 [pii]
AID - 10.1016/j.jss.2008.12.021 [doi]
PST - ppublish
SO  - J Surg Res. 2010 Jun 15;161(2):301-11. doi: 10.1016/j.jss.2008.12.021. Epub 2009 
      Jan 14.