PMID- 19482026
OWN - NLM
STAT- MEDLINE
DCOM- 20090915
LR  - 20181201
IS  - 1528-0012 (Electronic)
IS  - 0016-5085 (Linking)
VI  - 137
IP  - 3
DP  - 2009 Sep
TI  - Glucagon-like peptide-2 increases intestinal lipid absorption and chylomicron 
      production via CD36.
PG  - 997-1005, 1005.e1-4
LID - 10.1053/j.gastro.2009.05.051 [doi]
AB  - BACKGROUND & AIMS: Excessive postprandial lipemia is a prevalent condition that 
      results from intestinal oversecretion of apolipoprotein B48 (apoB48)-containing 
      lipoproteins. Glucagon-like peptide-2 (GLP-2) is a gastrointestinal-derived 
      intestinotropic hormone that links nutrient absorption to intestinal structure 
      and function. We investigated the effects of GLP-2 on intestinal lipid absorption 
      and lipoprotein production. METHODS: Intestinal lipid absorption and chylomicron 
      production were quantified in hamsters, wild-type mice, and Cd36(-/-) mice 
      infused with exogenous GLP-2. Newly synthesized apoB48 was metabolically labelled 
      in primary hamster jejunal fragments. Fatty acid absorption was measured, and 
      putative fatty acid transporters were assessed by immunoblotting. RESULTS: Human 
      GLP-2 increased secretion of the triglyceride (TG)-rich lipoprotein (TRL)-apoB48 
      following oral administration of olive oil to hamsters; TRL and cholesterol mass 
      each increased 3-fold. Fast protein liquid chromatography profiling indicated 
      that GLP-2 stimulated secretion of chylomicron/very low-density lipoprotein-sized 
      particles. Moreover, GLP-2 directly stimulated apoB48 secretion in jejunal 
      fragments cultured ex vivo, increased expression of fully glycosylated cluster of 
      differentiation 36/fatty acid translocase (CD36), and induced intestinal 
      absorption of [(3)H]triolein. The ability of GLP-2 to increase intestinal 
      lipoprotein production was lost in Cd36(-/-) mice. CONCLUSIONS: GLP-2 stimulates 
      intestinal apoB48-containing lipoprotein secretion, possibly through increased 
      lipid uptake, via a pathway that requires CD36. These findings suggest that GLP-2 
      represents a nutrient-dependent signal that regulates intestinal lipid absorption 
      and the assembly and secretion of TRLs from intestinal enterocytes.
FAU - Hsieh, Joanne
AU  - Hsieh J
AD  - Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, 
      Canada.
FAU - Longuet, Christine
AU  - Longuet C
FAU - Maida, Adriano
AU  - Maida A
FAU - Bahrami, Jasmine
AU  - Bahrami J
FAU - Xu, Elaine
AU  - Xu E
FAU - Baker, Christopher L
AU  - Baker CL
FAU - Brubaker, Patricia L
AU  - Brubaker PL
FAU - Drucker, Daniel J
AU  - Drucker DJ
FAU - Adeli, Khosrow
AU  - Adeli K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090529
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Apolipoprotein B-48)
RN  - 0 (CD36 Antigens)
RN  - 0 (Chylomicrons)
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fatty Acid Transport Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 0 (Lipoproteins)
RN  - 0 (Triglycerides)
RN  - 0 (lipoprotein triglyceride)
RN  - 122-32-7 (Triolein)
SB  - IM
CIN - Gastroenterology. 2009 Sep;137(3):775-8. PMID: 19643192
MH  - Animals
MH  - Apolipoprotein B-48/blood/metabolism
MH  - CD36 Antigens/metabolism
MH  - Chylomicrons/*metabolism
MH  - Cricetinae
MH  - Dietary Fats, Unsaturated/administration & dosage
MH  - Fatty Acid Transport Proteins/metabolism
MH  - Fatty Acids/metabolism
MH  - Glucagon-Like Peptide 2/pharmacology/*physiology
MH  - Intestinal Absorption/drug effects/*physiology
MH  - Jejunum/*metabolism
MH  - *Lipid Metabolism
MH  - Lipoproteins/chemistry/*metabolism
MH  - Male
MH  - Mesocricetus
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Triglycerides/chemistry/*metabolism
MH  - Triolein/metabolism
EDAT- 2009/06/02 09:00
MHDA- 2009/09/16 06:00
CRDT- 2009/06/02 09:00
PHST- 2008/09/17 00:00 [received]
PHST- 2009/05/13 00:00 [revised]
PHST- 2009/05/20 00:00 [accepted]
PHST- 2009/06/02 09:00 [entrez]
PHST- 2009/06/02 09:00 [pubmed]
PHST- 2009/09/16 06:00 [medline]
AID - S0016-5085(09)00868-3 [pii]
AID - 10.1053/j.gastro.2009.05.051 [doi]
PST - ppublish
SO  - Gastroenterology. 2009 Sep;137(3):997-1005, 1005.e1-4. doi: 
      10.1053/j.gastro.2009.05.051. Epub 2009 May 29.