PMID- 19484991
OWN - NLM
STAT- MEDLINE
DCOM- 20110519
LR  - 20090602
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 44
IP  - 1
DP  - 2009 Jan
TI  - [Expression and clinical significance of hypoxia-inducible factor-1alpha and 
      cyclooxygenase-2 in laryngeal squamous cell carcinoma].
PG  - 57-62
AB  - OBJECTIVE: To investigate the expression of hypoxia-inducible factor-1alpha 
      (HIF-1alpha) and cyclooxygenase-2 (COX-2) in laryngeal squamous cell carcinoma 
      (LSCC), while determine their relationship with clinicopathologic characteristics 
      and prognosis. METHODS: Tumor tissues were obtained from 60 patients who 
      underwent resection of laryngeal carcinoma in Affiliated First People's Hospital 
      of Shanghai Jiaotong University. Immunohistochemistry (Envision DAKO) was used to 
      detect the expressions of HIF-1alpha and COX-2 in the tumor tissues. As control 
      group, 15 cases of atypical hyperplasia, 10 cases of leukoplakia of vocal cord 
      and 10 cases of polyp of vocal cord were studied. All patients were regularly 
      followed up and the clinical data were collected systematically. RESULTS: 
      Positive staining rates of HIF-1alpha and COX-2 were 95.0% (57/60) and 98.3% 
      (59/60), respectively in all 60 specimen of LSCC. The positive expressions in 
      LSCC were significantly higher than those in atypical hyperplasia, leukoplakia 
      and polyp of vocal cord ( Fisher's exact test, P < 0.01). The expression of 
      HIF-1alpha was correlated with COX-2 in LSCC (r = 0.526, P < 0.01). High level 
      expressions of HIF-1alpha and COX-2 were 35.0% (21/60) and 38.3% (23/60) 
      respectively. High level expression of HIF-1alpha was significantly correlated 
      with clinical stages (chi2 = 4.331, P < 0.05) and lymph nodes metastases 
      (Fisher's exact test, P < 0.05). High level expression of COX-2 was significantly 
      correlated with clinical stage (chi2 = 8.539, P < 0.01) and T stages (chi2 = 
      6.792, P < 0.01). With univariate analysis, high level expressions of HIF-1alpha 
      and COX-2 were significantly associated with a worse overall survival (chi2 = 
      6.003, P < 0.05 and chi2 = 9.489, P < 0.01, respectively) and disease-free 
      survival (chi2 = 5.010, P < 0.05 and chi2 = 6.102, P < 0.05, respectively). With 
      multivariate analysis, recurrence and high level expression of COX-2 were two 
      unfavorable prognostic factors (RR = 7.104, P = 0.003; RR = 5.714, P = 0.008). 
      CONCLUSIONS: The expressions of HIF-1alpha and COX-2 played an important role in 
      the process of tumorigenesis and development of LSCC, The expression of 
      HIF-1alpha was correlated with COX-2 in LSCC. COX-2 and recurrence were probably 
      significant risk factors for prognosis of LSCC.
FAU - Xu, Cheng-zhi
AU  - Xu CZ
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Affiliated First 
      People's Hospital of Shanghai Jiaotong University, Shanghai 200080, China.
FAU - Dong, Pin
AU  - Dong P
FAU - Li, Xiao-yan
AU  - Li XY
FAU - Zhang, Zhi-jie
AU  - Zhang ZJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cyclooxygenase 2/*metabolism
MH  - Female
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Survival Analysis
EDAT- 2009/06/03 09:00
MHDA- 2011/05/20 06:00
CRDT- 2009/06/03 09:00
PHST- 2009/06/03 09:00 [entrez]
PHST- 2009/06/03 09:00 [pubmed]
PHST- 2011/05/20 06:00 [medline]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Jan;44(1):57-62.